{"id":2944,"date":"2023-11-15T06:43:14","date_gmt":"2023-11-15T06:43:14","guid":{"rendered":"https:\/\/oligox.abtvn.com\/?p=2944"},"modified":"2023-12-01T03:19:41","modified_gmt":"2023-12-01T03:19:41","slug":"tong-hop-oligonucleotide-vung-abasic-sites","status":"publish","type":"post","link":"https:\/\/oligox.abtvn.com\/en\/tong-hop-oligonucleotide-vung-abasic-sites\/","title":{"rendered":"T\u1ed4NG H\u1ee2P OLIGONUCLEOTIDE C\u00d3 CH\u1ee8A V\u00d9NG ABASIC SITE<\/strong>"},"content":{"rendered":"

Ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p m\u1edbi \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n \u0111\u1ec3 gi\u00fap oligonucleotide c\u00f3 v\u00f9ng Abasic site \u1ed5n \u0111\u1ecbnh t\u1ea1i b\u1ea5t k\u1ef3 v\u1ecb tr\u00ed n\u00e0o trong chu\u1ed7i khi s\u1eed d\u1ee5ng tetrahydrofuran bi\u1ebfn \u0111\u1ed5i, c\u00f3 c\u1ea5u tr\u00fac gi\u1ed1ng v\u1edbi 2′-deoxyribofuranose, nh\u01b0 m\u1ed9t ph\u00e2n t\u1eed t\u01b0\u01a1ng \u0111\u1ed3ng v\u1edbi c\u1ea5u tr\u00fac abasic site.<\/p>\n\n\n\n

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Khi di\u1ec5n ra qu\u00e1 tr\u00ecnh th\u1ee7y ph\u00e2n c\u00e1c nh\u00f3m nucleotide trong DNA c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn t\u00ecnh tr\u1ea1ng nucleotide b\u1ecb kh\u1eed base nit\u01a1 v\u00e0 h\u00ecnh th\u00e0nh c\u00e1c v\u00f9ng abasic site (v\u1ecb tr\u00ed trong DNA kh\u00f4ng c\u00f3 g\u1ed1c purine ho\u1eb7c pyrimidine). C\u1ee5 th\u1ec3, th\u00f4ng th\u01b0\u1eddng t\u1ea1i c\u00e1c v\u1ecb tr\u00ed dA (nu A v\u00e0 nu G) s\u1ebd d\u1ec5 b\u1ecb th\u1ee7y ph\u00e2n v\u00e0 g\u00e2y ra hi\u1ec7n t\u01b0\u1ee3ng t\u00e1ch kh\u1eed purine (depurination), h\u00ecnh th\u00e0nh v\u00f9ng abasic site. M\u1eb7c d\u00f9 qu\u00e1 tr\u00ecnh n\u00e0y di\u1ec5n ra ch\u1eadm, nh\u01b0ng khi \u1edf \u0111i\u1ec1u ki\u1ec7n m\u00f4i tr\u01b0\u1eddng acid (pH th\u1ea5p), ph\u1ea3n \u1ee9ng s\u1ebd x\u1ea3y ra nhanh h\u01a1n v\u00e0 \u0111\u1eb7c bi\u1ec7t l\u00e0 khi c\u00e1c baz\u01a1 \u0111\u00e3 b\u1ecb oxy h\u00f3a. C\u00e1c base b\u1ecb t\u1ed5n th\u01b0\u01a1ng n\u00e0y c\u0169ng c\u00f3 th\u1ec3 b\u1ecb lo\u1ea1i b\u1ecf b\u1edfi enzyme th\u00f4ng qua ho\u1ea1t \u0111\u1ed9ng c\u1ee7a DNA N-glycosylases (Base Excision Repair). <\/p>\n\n\n\n

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Abasic residue (dR)<\/strong> t\u1ed3n t\u1ea1i ch\u1ee7 y\u1ebfu d\u01b0\u1edbi d\u1ea1ng chu k\u1ef3 v\u00e0 c\u1ea5u tr\u00fac c\u1ee7a n\u00f3 \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb trong H\u00ecnh 1<\/em><\/strong>. Tr\u1ea1ng th\u00e1i v\u00f9ng abasic site th\u01b0\u1eddng th\u1ec3 hi\u1ec7n t\u00ednh \u1ed5n \u0111\u1ecbnh k\u00e9m, \u0111\u1eb7c bi\u1ec7t l\u00e0 \u1edf m\u00f4i tr\u01b0\u1eddng baz\u01a1. \u0110i\u1ec1u n\u00e0y l\u00e0 do t\u00ednh kh\u00f4ng \u1ed5n \u0111\u1ecbnh c\u1ee7a c\u1ea5u tr\u00fac aldehyde, tautomeric c\u1ee7a chu k\u1ef3 \u0111\u1ed1i v\u1edbi qu\u00e1 tr\u00ecnh \u00df-elimination. Ph\u1ea3n \u1ee9ng ph\u00e2n h\u1ee7y n\u00e0y d\u1eabn \u0111\u1ebfn ph\u00e2n c\u1eaft chu\u1ed7i (chain scission) t\u1ea1i v\u00f9ng abasic site v\u1edbi s\u1ef1 h\u00ecnh th\u00e0nh v\u00f9ng 5\u2019 phosphate bi\u1ebfn \u0111\u1ed5i (5′-phosphate segment) v\u00e0 v\u00f9ng 3′ bi\u1ebfn \u0111\u1ed5i (3′-modified segment).<\/p>\n\n\n\n

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Do t\u00ednh kh\u00f4ng \u1ed5n \u0111\u1ecbnh c\u1ee7a v\u00f9ng abasic residue, vi\u1ec7c t\u1ea1o ra bi\u1ebfn th\u1ec3 n\u00e0y th\u00f4ng qua ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p h\u00f3a h\u1ecdc tr\u1edf n\u00ean kh\u00f3 th\u1ef1c hi\u1ec7n. Tuy nhi\u00ean, m\u1ed9t s\u1ed1 b\u00e0i b\u00e1o thu nh\u1eadn k\u1ebft qu\u1ea3 t\u1ed1t khi s\u1eed d\u1ee5ng tetrahydrofuran bi\u1ebfn \u0111\u1ed5i nh\u01b0 dSpacer tetrahydrofuran analogue gi\u00fap \u1ed5n \u0111\u1ecbnh ph\u1ea3n \u1ee9ng. Bi\u1ebfn th\u1ec3 (H\u00ecnh 1) <\/em>thi\u1ebfu g\u1ed1c 1′-OH c\u1ee7a dR mang t\u00ednh \u1ed5n \u0111\u1ecbnh trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p, tinh s\u1ea1ch oligo v\u00e0 l\u01b0u tr\u1eef. Ch\u00fang c\u00f3 kh\u1ea3 n\u0103ng t\u1ed5ng h\u1ee3p v\u00f9ng abasic residue b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng N-uracil glycosylase \u0111\u1ec3 lo\u1ea1i b\u1ecf uracil t\u1eeb 2′-deoxyuridine residue. <\/p>\n\n\n\n

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Trong ph\u01b0\u01a1ng ph\u00e1p h\u00f3a h\u1ecdc t\u1eeb Rayner, abasic site \u0111\u01b0\u1ee3c b\u1ea3o v\u1ec7 b\u1eb1ng m\u1ed9t nh\u00f3m photolabile 2-nitrobenzyl (H\u00ecnh 1)<\/em> trong su\u1ed1t qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p v\u00e0 tinh s\u1ea1ch oligo. Nh\u00f3m 2-nitrobenzyl sau \u0111\u00f3 \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf th\u00f4ng qua ph\u1ea3n \u1ee9ng quang ph\u00e2n (photolysis) \u0111\u1ec3 t\u1ea1o ra abasic site. Tuy nhi\u00ean, c\u1ea7n l\u01b0u \u00fd v\u1ec1 vi\u1ec7c h\u00ecnh th\u00e0nh thymine dimer trong qu\u00e1 tr\u00ecnh n\u00e0y. M\u1ed9t s\u1ed1 ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u1ed5ng h\u1ee3p v\u00f9ng abasic site, nh\u01b0ng cho \u0111\u1ebfn nay, qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p monomer v\u1eabn c\u00f2n \u0111ang \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u.<\/p>\n\n\n\n

\"C\u1ea5u
H\u00ecnh 1. <\/em><\/strong>C\u1ea5u tr\u00fac s\u1ea3n ph\u1ea9m<\/em>.
(Ngu\u1ed3n: Glen Research)<\/figcaption><\/figure>\n\n\n\n
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1. T\u1ed5ng h\u1ee3p Oligonucleotide v\u00f9ng abasic site<\/strong><\/h2>\n\n\n\n

M\u1ed9t ph\u01b0\u01a1ng ph\u00e1p h\u00f3a h\u1ecdc \u0111\u01b0\u1ee3c nh\u1eafc \u0111\u1ebfn khi t\u1ed5ng h\u1ee3p c\u00e1c oligonucleotide m\u1ea1ch \u0111\u01a1n v\u00e0 \u0111\u00f4i c\u00f3 ch\u1ee9a v\u00f9ng abasic site b\u1eb1ng \u0111i\u1ec1u ki\u1ec7n kh\u00f4ng qu\u00e1 g\u1eaft gao v\u00e0 kh\u1ea3 n\u0103ng cho ra c\u00e1c ph\u1ea3n \u1ee9ng ph\u1ee5 r\u1ea5t th\u1ea5p. Trong qu\u00e1 tr\u00ecnh n\u00e0y, h\u1ee3p ch\u1ea5t 3-deoxyhexitol \u0111\u01b0\u1ee3c d\u00f9ng nh\u01b0 m\u1ed9t monomer (H\u00ecnh 1<\/em>), \u0111\u01b0\u1ee3c b\u1ea3o v\u1ec7 \u0111\u1ec3 ng\u0103n ch\u1eb7n ph\u1ea3n \u1ee9ng kh\u00f4ng mong mu\u1ed1n. <\/p>\n\n\n\n

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Sau khi t\u1ed5ng h\u1ee3p oligo theo \u0111i\u1ec1u ki\u1ec7n ti\u00eau chu\u1ea9n, c\u00e1c nh\u00f3m b\u1ea3o v\u1ec7 silyl c\u1ee7a v\u00f9ng (h\u00ecnh 2) \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf b\u1eb1ng axit n\u01b0\u1edbc. (\u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 th\u1ef1c hi\u1ec7n c\u00f9ng v\u1edbi qu\u00e1 tr\u00ecnh lo\u1ea1i b\u1ecf trityl trong b\u01b0\u1edbc cu\u1ed1i c\u00f9ng c\u1ee7a qu\u00e1 tr\u00ecnh tinh s\u1ea1ch b\u1eb1ng DMT-ON.) Ch\u00fang t\u1ea1o ra m\u1ed9t d\u1ea1ng diol (h\u00ecnh 2), sau \u0111\u00f3 \u0111\u01b0\u1ee3c x\u1eed l\u00fd v\u1edbi sodium periodate \u0111\u1ec3 t\u1ea1o th\u00e0nh aldehyde v\u00e0 formaldehyde. Aldehyde n\u00e0y sau \u0111\u00f3 ngay l\u1eadp t\u1ee9c xo\u1eafn th\u00e0nh m\u1ed9t c\u1ea5u tr\u00fac m\u1edbi – \u0111\u01b0\u1eddng abasic cyclic sugar (dR).<\/p>\n\n\n\n

\"Q\u00faa
H\u00ecnh 2. <\/em><\/strong>Qu\u00e1 tr\u00ecnh h\u00ecnh th\u00e0nh abasic site c\u1ee7a Oligonucleotide<\/em>. (Ngu\u1ed3n: Glen Research)<\/figcaption><\/figure>\n\n\n\n

2. T\u00ednh \u1ed5n \u0111\u1ecbnh c\u1ee7a oligonucleotide<\/strong><\/h2>\n\n\n\n

V\u1edbi s\u1ef1 xu\u1ea5t hi\u1ec7n c\u1ee7a oligonucleotide ch\u1ee9a v\u00f9ng abasic site, \u0111\u01b0\u1ee3c ch\u1ee9ng minh l\u00e0 \u1ed5n \u0111\u1ecbnh trong dung d\u1ecbch \u0111\u1ec7m axetat triethylammonium buffer 0.2M (pH6) \u1edf 5\u00b0C ho\u1eb7c th\u1ea5p h\u01a1n. Tuy nhi\u00ean, tr\u1ea1ng th\u00e1i n\u00e0y \u00edt \u1ed5n \u0111\u1ecbnh \u1edf 55\u00b0C (kho\u1ea3ng 7 gi\u1edd) v\u00e0 k\u00e9m h\u01a1n khi \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (kho\u1ea3ng 30 ng\u00e0y). \u0110\u00e1ng ch\u00fa \u00fd, abasic site b\u1ecb ph\u00e2n h\u1ee7y ho\u00e0n to\u00e0n trong qu\u00e1 tr\u00ecnh bay h\u01a1i cho \u0111\u1ebfn khi \u0111\u00f4ng kh\u00f4.<\/p>\n\n\n\n

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3. \u0110\u1eb7c \u0111i\u1ec3m c\u1ea5u tr\u00fac Oligonucleotide<\/strong><\/h2>\n\n\n\n

\u0110\u1ed1i v\u1edbi \u0111\u1eb7c \u0111i\u1ec3m c\u1ea5u tr\u00fac, c\u00e1c nghi\u00ean c\u1ee9u v\u1ec1 t\u00ednh n\u00f3ng ch\u1ea3y c\u1ee7a oligo ch\u1ee9a abasic site \u0111\u00e3 \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh. K\u1ebft qu\u1ea3 cho th\u1ea5y r\u1eb1ng abasic site kh\u00f4ng t\u1ea1o th\u00e0nh c\u1eb7p \u1ed5n \u0111\u1ecbnh v\u1edbi b\u1ea5t k\u1ef3 trong 4 base n\u00e0o (adenine, thymine, cytosine, v\u00e0 guanine) tr\u00ean chu\u1ed7i b\u1ed5 sung, t\u01b0\u01a1ng t\u1ef1 v\u1edbi dSpacer. Nghi\u00ean c\u1ee9u c\u0169ng \u0111\u00e1nh gi\u00e1 c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m v\u1eadt l\u00fd kh\u00e1c v\u00e0 m\u1ed1i li\u00ean quan c\u1ee7a ch\u00fang \u0111\u1ed1i v\u1edbi t\u1ed5n th\u01b0\u01a1ng v\u00e0 s\u1eeda ch\u1eefa DNA.<\/p>\n\n\n\n

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Pannel \u1ee9ng d\u1ee5ng trong ch\u1ea9n \u0111o\u00e1n Thalassemia<\/a><\/h3>
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T\u1ed5ng quan Thalassemia Gi\u1edbi thi\u1ec7u Thalassemia Thalassemia (hay c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi v\u1edbi t\u00ean b\u1ec7nh tan m\u00e1u b\u1ea9m sinh) l\u00e0 m\u1ed9t b\u1ec7nh l\u00fd huy\u1ebft h\u1ecdc di truy\u1ec1n c\u00f3 t\u1ef7 l\u1ec7 l\u01b0u h\u00e0nh cao tr\u00ean to\u00e0n c\u1ea7u v\u00e0 l\u00e0 m\u1ed9t v\u1ea5n \u0111\u1ec1…<\/p>\n<\/div>

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\u1ea2nh h\u01b0\u1edfng c\u1ee7a TaqMan probe \u0111\u1ebfn \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9 nh\u1ea1y trong Realtime PCR<\/a><\/h3>
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TaqMan Probe \u0111\u00f3ng vai tr\u00f2 trung t\u00e2m trong vi\u1ec7c \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 ch\u00ednh x\u00e1c, \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a ph\u1ea3n \u1ee9ng trong k\u1ef9 thu\u1eadt Real-time PCR<\/p>\n<\/div>

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TAQMAN PROBE V\u00c0 C\u00c1C TI\u00caU CH\u00cd QUAN TR\u1eccNG KHI THI\u1ebeT K\u1ebe PROBE TRONG REAL TIME PCR<\/a><\/h3>
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Probe (\u0111\u1ea7u d\u00f2) trong sinh h\u1ecdc ph\u00e2n t\u1eed l\u00e0 m\u1ed9t \u0111o\u1ea1n oligonucleotide (DNA ho\u1eb7c RNA s\u1ee3i \u0111\u01a1n) ng\u1eafn, \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng c\u00e1c ph\u00e2n t\u1eed ph\u00e1t t\u00edn hi\u1ec7u (th\u01b0\u1eddng l\u00e0 c\u00e1c ch\u1ea5t ph\u00e1t hu\u1ef3nh quang). Probe \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3…<\/p>\n<\/div>

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HPLC L\u00e0 G\u00ec? \u1ee8ng D\u1ee5ng C\u1ee7a HPLC Trong Tinh S\u1ea1ch v\u00e0 Ph\u00e2n T\u00edch Oligonucleotide<\/a><\/h3>
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HPLC (High Performance Liquid Chromatography) \u2013 S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch, \u0111\u1ecbnh t\u00ednh ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng c\u00e1c ch\u1ea5t, th\u00e0nh ph\u1ea7n trong m\u1ed9t h\u1ed7n h\u1ee3p ch\u1ea5t l\u1ecfng.<\/p>\n<\/div>

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C\u00c1C PH\u01af\u01a0NG PH\u00c1P TINH S\u1ea0CH OLIGONUCLEOTIDE<\/a><\/h3>
\n \n \n<\/svg>\nadmin<\/a><\/span>\n \n<\/svg>\nJan 15, 2025<\/span>\n \n \n<\/svg>\n9 min read<\/span><\/div>

Oligonucleotide l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA m\u1ea1ch \u0111\u01a1n ho\u1eb7c m\u1ea1ch \u0111\u00f4i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed, y h\u1ecdc v\u00e0 nghi\u00ean c\u1ee9u khoa h\u1ecdc. C\u00e1c tr\u00ecnh t\u1ef1 oligonucleotide…<\/p>\n<\/div>

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D\u1ecbch v\u1ee5 OligoX<\/a><\/em><\/strong><\/h3>

T\u1ed5ng h\u1ee3p Oligonucleotide bao g\u1ed3m c\u00e1c s\u1ea3n ph\u1ea9m PCR, qPCR Primer, Primer\/Probe bi\u1ebfn \u0111\u1ed5i, Tr\u00ecnh t\u1ef1 RNA, Amplicon v\u00e0 c\u00e1c t\u1ed5ng h\u1ee3p \u0111\u1eb7c bi\u1ec7t<\/p><\/div>

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Ngu\u1ed3n tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n
    \n
  1. Glen Research. https:\/\/www.glenresearch.com\/reports\/gr14-13<\/a><\/li>\n\n\n\n
  2. M. Takeshita, C.N. Chang, F. Johnson, S. Will, and A.P. Grollman, J. Biol. Chem., 1987, 262, 10171-10179. https:\/\/pubmed.ncbi.nlm.nih.gov\/2440861\/<\/a><\/li>\n\n\n\n
  3. M.W. Kalnik, C.N. Chang, A.P. Grollman, and D.J. Patel, Biochemistry, 1988, 27, 924-931.<\/li>\n\n\n\n
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  6. <\/li>\n\n\n\n
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  11. J. Lhomme, J.F. Constant, and M. Demeunynck, Biopolymers, 1999, 52, 65-83.<\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"

    Ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p m\u1edbi \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n \u0111\u1ec3 gi\u00fap oligonucleotide c\u00f3 v\u00f9ng Abasic site \u1ed5n \u0111\u1ecbnh t\u1ea1i b\u1ea5t k\u1ef3 v\u1ecb tr\u00ed n\u00e0o trong chu\u1ed7i khi s\u1eed d\u1ee5ng tetrahydrofuran bi\u1ebfn \u0111\u1ed5i, c\u00f3 c\u1ea5u tr\u00fac gi\u1ed1ng v\u1edbi 2′-deoxyribofuranose, nh\u01b0 m\u1ed9t ph\u00e2n t\u1eed t\u01b0\u01a1ng \u0111\u1ed3ng v\u1edbi c\u1ea5u tr\u00fac abasic site. Khi di\u1ec5n ra qu\u00e1 tr\u00ecnh th\u1ee7y …<\/p>\n

    T\u1ed4NG H\u1ee2P OLIGONUCLEOTIDE C\u00d3 CH\u1ee8A V\u00d9NG ABASIC SITE<\/strong><\/span> Read More \u00bb<\/a><\/p>","protected":false},"author":1,"featured_media":3061,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[27],"tags":[29,30],"class_list":["post-2944","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-updated-news","tag-oligonucleotide","tag-updated-news"],"jetpack_featured_media_url":"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site.png","uagb_featured_image_src":{"full":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site.png",3840,2160,false],"thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-150x150.png",150,150,true],"medium":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-300x169.png",300,169,true],"medium_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-768x432.png",768,432,true],"large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-1024x576.png",1024,576,true],"1536x1536":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-1536x864.png",1536,864,true],"2048x2048":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-2048x1152.png",2048,1152,true],"trp-custom-language-flag":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-18x10.png",18,10,true],"ultp_layout_landscape_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-1200x800.png",1200,800,true],"ultp_layout_landscape":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-870x570.png",870,570,true],"ultp_layout_portrait":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-600x900.png",600,900,true],"ultp_layout_square":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-600x600.png",600,600,true],"woocommerce_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-300x300.png",300,300,true],"woocommerce_single":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-600x338.png",600,338,true],"woocommerce_gallery_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/11\/Tong-hop-oligonucleotide-vung-abasic-site-100x100.png",100,100,true]},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/oligox.abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p m\u1edbi \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n \u0111\u1ec3 gi\u00fap oligonucleotide c\u00f3 v\u00f9ng Abasic site \u1ed5n \u0111\u1ecbnh t\u1ea1i b\u1ea5t k\u1ef3 v\u1ecb tr\u00ed n\u00e0o trong chu\u1ed7i khi s\u1eed d\u1ee5ng tetrahydrofuran bi\u1ebfn \u0111\u1ed5i, c\u00f3 c\u1ea5u tr\u00fac gi\u1ed1ng v\u1edbi 2′-deoxyribofuranose, nh\u01b0 m\u1ed9t ph\u00e2n t\u1eed t\u01b0\u01a1ng \u0111\u1ed3ng v\u1edbi c\u1ea5u tr\u00fac abasic site. 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