{"id":3154,"date":"2023-12-08T07:26:51","date_gmt":"2023-12-08T07:26:51","guid":{"rendered":"https:\/\/oligox.abtvn.com\/?p=3154"},"modified":"2025-08-01T03:24:54","modified_gmt":"2025-08-01T03:24:54","slug":"danh-dau-oligo-va-dna-bang-click-chemistry","status":"publish","type":"post","link":"https:\/\/oligox.abtvn.com\/en\/danh-dau-oligo-va-dna-bang-click-chemistry\/","title":{"rendered":"Labeling Oligonucleotides and DNA Using Click Chemistry Techniques<\/strong>"},"content":{"rendered":"

Click chemistry<\/strong> l\u00e0 m\u1ed9t ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc \u0111\u01b0\u1ee3c gi\u1edbi thi\u1ec7u l\u1ea7n \u0111\u1ea7u ti\u00ean v\u00e0o n\u0103m 2001, b\u1edfi nh\u00e0 khoa h\u1ecdc Sharpless v\u00e0 c\u1ed9ng s\u1ef1. Trong \u0111\u00f3, ph\u1ea3n \u1ee9ng click nh\u1ea5n m\u1ea1nh t\u1ea7m quan tr\u1ecdng c\u1ee7a vi\u1ec7c s\u1eed d\u1ee5ng c\u00e1c \u0111i\u1ec1u ki\u1ec7n ph\u1ea3n \u1ee9ng \u0111\u01a1n gi\u1ea3n \u0111\u1ec3 li\u00ean k\u1ebft c\u00e1c \u0111\u01a1n v\u1ecb, ph\u00e2n t\u1eed nh\u1ecf v\u1edbi nhau m\u1ed9t c\u00e1ch nhanh ch\u00f3ng, \u0111\u00e1ng tin c\u1eady nh\u1eb1m b\u1eaft ch\u01b0\u1edbc ph\u01b0\u01a1ng ph\u00e1p t\u1ea1o ra s\u1ef1 \u0111a d\u1ea1ng ph\u00e2n t\u1eed c\u1ee7a t\u1ef1 nhi\u00ean. <\/p>\n\n\n\n

Hi\u1ec7n nay, ch\u00fang \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn l\u00e0 m\u1ed9t t\u1eadp h\u1ee3p c\u00e1c ph\u1ea3n \u1ee9ng ch\u1ecdn l\u1ecdc, hi\u1ec7u qu\u1ea3 cao, ch\u00ednh x\u00e1c v\u00e0 nhanh ch\u00f3ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c h\u1ee3p ch\u1ea5t ti\u1ec1m n\u0103ng \u1ee9ng d\u1ee5ng trong c\u00e1c d\u01b0\u1ee3c ph\u1ea9m nh\u01b0 c\u00e1c ch\u1ea5t kh\u00e1ng virus, kh\u00e1ng khu\u1ea9n v\u00e0 \u1ee9ng d\u1ee5ng trong chu\u1ea9n \u0111o\u00e1n, h\u00ecnh \u1ea3nh y sinh. N\u0103m 2022, ph\u1ea3n \u1ee9ng Click \u0111\u00e3 xu\u1ea5t s\u1eafc gi\u00e0nh \u0111\u01b0\u1ee3c gi\u1ea3i th\u01b0\u1edfng nobel h\u00f3a h\u1ecdc danh gi\u00e1 v\u1edbi nhi\u1ec1u \u1ee9ng d\u1ee5ng trong c\u00e1c l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u ph\u00e1t tri\u1ec3n d\u01b0\u1ee3c ph\u1ea9m v\u00e0 l\u1eadp b\u1ea3n \u0111\u1ed3 ADN.<\/p>\n\n\n\n

Ph\u1ea3n \u1ee9ng Click \u0111\u01b0\u1ee3c chia th\u00e0nh 4 lo\u1ea1i ch\u00ednh bao g\u1ed3m: Ph\u1ea3n \u1ee9ng c\u1ed9ng v\u00f2ng (Cycloaddtion), ph\u1ea3n \u1ee9ng m\u1edf v\u00f2ng nucleophilic (Nucleophilic substitution), ph\u1ea3n \u1ee9ng ng\u01b0ng t\u1ef1 Carbonyl (Carbonyl condensation), v\u00e0 ph\u1ea3n \u1ee9ng c\u1ed9ng.  Trong s\u1ed1 4 lo\u1ea1i ch\u00ednh, ph\u1ea3n \u1ee9ng c\u1ed9ng v\u00f2ng, \u0111\u1eb7c bi\u1ec7t l\u00e0 ph\u1ea3n \u1ee9ng c\u1ed9ng v\u00f2ng tu\u1ea7n ho\u00e0n 1,3 l\u01b0\u1ee1ng c\u1ef1c c\u1ee7a nh\u00e0 nghi\u00ean c\u1ee9u Huisgen, \u0111\u00e3 \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn v\u00e0 s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i nhi\u1ec1u nh\u1ea5t tr\u00ean th\u1ebf gi\u1edbi. D\u1ef1a tr\u00ean c\u00e1c c\u00f4ng b\u1ed1 nghi\u00ean c\u1ee9u khoa h\u1ecdc \u0111\u01b0\u1ee3c \u0111\u1ec1 c\u1eadp xu\u1ea5t b\u1ea3n tr\u01b0\u1edbc \u0111\u00f3, g\u1ea7n 100% c\u00e1c \u1ea5n ph\u1ea9m \u0111\u1ec1u \u0111\u1ec1 c\u1eadp \u0111\u1ebfn ph\u1ea3n \u1ee9ng click n\u00e0y, ph\u1ea3n \u1ee9ng n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng tr\u00ean nhi\u1ec1u l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u \u0111a d\u1ea1ng. <\/p>\n\n\n\n

Ch\u00ednh v\u00ec v\u1eady trong b\u00e0i vi\u1ebft n\u00e0y s\u1ebd \u0111\u1ec1 c\u1eadp \u0111\u1ebfn lo\u1ea1i ph\u1ea3n \u1ee9ng click c\u1ed9ng v\u00f2ng Huisgen v\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1nh d\u1ea5u c\u00e1c oligonucleotide, DNA, peptide v\u00e0 protein b\u1eb1ng ph\u1ea3n \u1ee9ng click n\u00e0y.<\/p>\n\n\n\n

\"H\u00ecnh
H\u00ecnh 1. <\/strong>B\u1ed1n lo\u1ea1i c\u1ee7a ph\u1ea3n \u1ee9ng Click<\/em><\/figcaption><\/figure>\n\n\n\n

C\u01a1 ch\u1ebf c\u1ee7a ph\u1ea3n \u1ee9ng Click chemistry<\/strong><\/h2>\n\n\n\n

Ph\u1ea3n \u1ee9ng click l\u00e0 s\u1ef1 ph\u1ea3n \u1ee9ng gi\u1eefa ph\u00e2n t\u1eed azide v\u00e0 alkyne t\u1ea1o ra s\u1ea3n ph\u1ea9m c\u1ed9ng h\u00f3a tr\u1ecb -1,2,3-triazole. Qu\u00e1 tr\u00ecnh n\u00e0y c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 ph\u1ea3n \u1ee9ng c\u1ed9ng v\u00f2ng alkyne-azide \u0111\u01b0\u1ee3c x\u00fac t\u00e1c b\u1eb1ng CuAAC. Ch\u1ea5t x\u00fac t\u00e1c th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng d\u01b0\u1edbi d\u1ea1ng ph\u1ee9c h\u1ee3p ph\u1ed1i t\u1eed \u0111\u1ed3ng. Trong v\u00f4 s\u1ed1 nhi\u1ec1u ph\u1ea3n \u1ee9ng h\u1eefu c\u01a1, ph\u1ea3n \u1ee9ng click \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn l\u00e0m ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc li\u00ean h\u1ee3p v\u00ec m\u1ed9t s\u1ed1 \u01b0u \u0111i\u1ec3m.<\/p>\n\n\n\n

\"H\u00ecnh
H\u00ecnh 2.<\/strong> C\u01a1 ch\u1ebf c\u1ee7a ph\u1ea3n \u1ee9ng click<\/em><\/figcaption><\/figure>\n\n\n\n

\u01afu \u0111i\u1ec3m c\u1ee7a ph\u1ea3n \u1ee9ng click Chemistry<\/strong><\/h2>\n\n\n\n

Ph\u1ea3n \u1ee9ng click c\u00f3 t\u00ednh ch\u1ecdn l\u1ecdc. Ch\u00fang ch\u1ec9 di\u1ec5n ra gi\u1eefa c\u00e1c ph\u00e2n t\u1eed azide v\u00e0 alkyne. Ph\u1ea3n \u1ee9ng kh\u00f4ng b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi c\u00e1c nh\u00f3m h\u1eefu c\u01a1 kh\u00e1c c\u00f3 trong DNA v\u00e0 protein c\u1ea7n \u0111\u00e1nh d\u1ea5u, ch\u1eb3ng h\u1ea1n nh\u01b0 nh\u00f3m amino v\u00e0 carboxy.<\/p>\n\n\n\n

Azide v\u00e0 alkynes kh\u00f4ng hi\u1ec7n di\u1ec7n trong c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc t\u1ef1 nhi\u00ean. Nh\u1eefng nh\u00f3m n\u00e0y \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o DNA v\u00e0 protein m\u1ed9t c\u00e1ch \u0111\u1eb7c bi\u1ec7t. DNA ch\u1ee9a alkyne c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ebf b\u1eb1ng alkyne phosphoramidite trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p oligo c\u01a1 b\u1ea3n. C\u00e1c protein \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1edfi azide v\u00e0 alkyne c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1eb1ng c\u00e1ch ho\u1ea1t h\u00f3a este-azide ho\u1eb7c ho\u1ea1t h\u00f3a este-alkyne.<\/p>\n\n\n\n

Ph\u1ea3n \u1ee9ng click di\u1ec5n ra trong n\u01b0\u1edbc. Dung d\u1ecbch DMSO, DMF, acetonitrile, ethanol ho\u1eb7c n\u01b0\u1edbc tinh khi\u1ebft v\u00e0 buffer c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho ph\u1ea3n \u1ee9ng. Ph\u1ea3n \u1ee9ng c\u00f3 kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch sinh h\u1ecdc v\u00e0 c\u00f3 th\u1ec3 di\u1ec5n ra trong t\u1ebf b\u00e0o s\u1ed1ng.<\/p>\n\n\n\n

Ph\u01b0\u01a1ng th\u1ee9c (protocol) t\u1ed5ng h\u1ee3p m\u1ed9t c\u00e1ch \u0111\u01a1n gi\u1ea3n, \u00edt thao t\u00e1c, nhanh ch\u00f3ng.<\/p>\n\n\n\n

Ph\u1ea3n \u1ee9ng kh\u00f4ng nh\u1ea1y c\u1ea3m v\u1edbi pH. Kh\u00f4ng gi\u1ed1ng nh\u01b0 ph\u1ea3n \u1ee9ng c\u1ee7a NHS-ester v\u1edbi amin v\u00e0 m\u1ed9t s\u1ed1 ch\u1ea5t h\u00f3a h\u1ecdc li\u00ean h\u1ee3p kh\u00e1c, h\u1ed7n h\u1ee3p ph\u1ea3n \u1ee9ng kh\u00f4ng c\u1ea7n ki\u1ec3m so\u00e1t \u0111\u1ed9 pH. Kh\u00f4ng c\u1ea7n \u0111i\u1ec1u ch\u1ec9nh buffer b\u1eb1ng acid ho\u1eb7c base \u2013 ph\u1ea3n \u1ee9ng Click ho\u1ea1t \u0111\u1ed9ng t\u1ed1t trong kho\u1ea3ng pH t\u1eeb 4-11.<\/p>\n\n\n\n

Do \u0111\u00f3, k\u1ef9 thu\u1eadt n\u00e0y \u0111\u00e3 tr\u1edf th\u00e0nh m\u1ed9t k\u1ef9 thu\u1eadt ph\u1ed5 bi\u1ebfn \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 chu\u1ea9n b\u1ecb t\u1ed5ng h\u1ee3p n\u00ean c\u00e1c DNA, peptide, protein, oligo bi\u1ebfn \u0111\u1ed5i \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u hu\u1ef3nh quang (probe).<\/p>\n\n\n\n

Ph\u01b0\u01a1ng th\u1ee9c \u0111\u00e1nh d\u1ea5u oligo (Probe) b\u1eb1ng k\u1ef9 thu\u1eadt click Chemistry<\/strong><\/h2>\n\n\n\n

\u0110\u00e1nh d\u1ea5u oligonucleotide b\u1eb1ng ph\u1ea3n \u1ee9ng click l\u00e0 m\u1ed9t ph\u1ea3n \u1ee9ng quy tr\u00ecnh g\u1ed3m 2 b\u01b0\u1edbc. \u0110\u1ea7u ti\u00ean, alkyne \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o oligonucleotide b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng alkyne amidite. Th\u1ee9 hai, oligo \u0111\u00e3 g\u1eafn alkynyl \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng thu\u1ed1c nhu\u1ed9m c\u00f3 ch\u1ee9a azide trong c\u00e1c \u0111i\u1ec1u ki\u1ec7n th\u00edch h\u1ee3p. Oligo \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u sau \u0111\u00f3 \u0111\u01b0\u1ee3c k\u1ebft t\u1ee7a b\u1eb1ng aceton v\u00e0 \u0111\u01b0\u1ee3c tinh s\u1ea1ch b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p ph\u00f9 h\u1ee3p. (HPLC l\u00e0 ph\u01b0\u01a1ng ph\u00e1p th\u00edch h\u1ee3p nh\u1ea5t).<\/p>\n\n\n\n

\"H\u00ecnh
H\u00ecnh 3. <\/strong>Ph\u01b0\u01a1ng th\u1ee9c \u0111\u00e1nh d\u1ea5u oligo b\u1eb1ng k\u1ef9 thu\u1eadt click<\/em><\/figcaption><\/figure>\n\n\n\n

So v\u1edbi vi\u1ec7c \u0111\u00e1nh d\u1ea5u d\u1ef1a tr\u00ean thu\u1ed1c nhu\u1ed9m amidite, ph\u1ea3n \u1ee9ng click t\u1ea1o ra c\u00e1c oligonucleotide \u0111\u00e1nh d\u1ea5u tinh khi\u1ebft h\u01a1n, hi\u1ec7u su\u1ea5t cao h\u01a1n, c\u1ea7n \u00edt tinh s\u1ea1ch h\u01a1n. Thu\u1ed1c nhu\u1ed9m \u0111\u1eaft ti\u1ec1n v\u00e0 nh\u1ea1y c\u1ea3m kh\u00f4ng c\u1ea7n s\u1eed d\u1ee5ng  ph\u1ea3n \u1ee9ng kh\u1eed (debocking reagents) v\u00e0 s\u1ebd kh\u00f4ng b\u1ecb l\u00e3ng ph\u00ed.<\/p>\n\n\n\n

So v\u1edbi ph\u01b0\u01a1ng ph\u00e1p NHS ester, ph\u1ea3n \u1ee9ng click c\u00f3 kh\u1ea3 n\u0103ng m\u1edf r\u1ed9ng, \u1ee9ng d\u1ee5ng nhi\u1ec1u h\u01a1n v\u00e0 c\u1ea7n \u00edt thu\u1ed1c nhu\u1ed9m h\u01a1n. C\u00e1c oligo \u0111\u00e1nh d\u1ea5u \u0111\u01b0\u1ee3c l\u00e0m s\u1ea1ch d\u1ec5 d\u00e0ng h\u01a1n. Ph\u1ea7n d\u01b0 c\u1ee7a thu\u1ed1c nhu\u1ed9m g\u1ea7n nh\u01b0 \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf ho\u00e0n to\u00e0n trong qu\u00e1 tr\u00ecnh k\u1ebft t\u1ee7a acetone. So v\u1edbi vi\u1ec7c \u0111\u00e1nh d\u1ea5u este, \u0111\u00f4i khi c\u1ea7n ph\u1ea3i k\u1ebft t\u1ee7a nhi\u1ec1u l\u1ea7n \u0111\u1ec3 lo\u1ea1i b\u1ecf thu\u1ed1c nhu\u1ed9m d\u01b0 th\u1eeba. T\u00ednh ch\u1ea5t ph\u1ea3n \u1ee9ng click cho ph\u00e9p \u0111\u00e1nh d\u1ea5u hi\u1ec7u qu\u1ea3 h\u00e0ng tr\u0103m \u0111\u01a1n v\u1ecb oligonucleotide.<\/p>\n\n\n\n

\"H\u00ecnh
H\u00ecnh 4. <\/strong>B\u1ea3ng \u01b0u nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1nh d\u1ea5u oligo<\/em><\/figcaption><\/figure>\n\n\n
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Pannel \u1ee9ng d\u1ee5ng trong ch\u1ea9n \u0111o\u00e1n Thalassemia<\/a><\/h3>
\n \n \n<\/svg>\nadmin<\/a><\/span>\n \n<\/svg>\nAug 26, 2025<\/span>\n \n \n<\/svg>\n18 min read<\/span><\/div>

T\u1ed5ng quan Thalassemia Gi\u1edbi thi\u1ec7u Thalassemia Thalassemia (hay c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi v\u1edbi t\u00ean b\u1ec7nh tan m\u00e1u b\u1ea9m sinh) l\u00e0 m\u1ed9t b\u1ec7nh l\u00fd huy\u1ebft h\u1ecdc di truy\u1ec1n c\u00f3 t\u1ef7 l\u1ec7 l\u01b0u h\u00e0nh cao tr\u00ean to\u00e0n c\u1ea7u v\u00e0 l\u00e0 m\u1ed9t v\u1ea5n \u0111\u1ec1…<\/p>\n<\/div>

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\n \n \n<\/svg>\nadmin<\/a><\/span>\n \n<\/svg>\nMay 27, 2025<\/span>\n \n \n<\/svg>\n26 min read<\/span><\/div>

TaqMan Probe \u0111\u00f3ng vai tr\u00f2 trung t\u00e2m trong vi\u1ec7c \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 ch\u00ednh x\u00e1c, \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a ph\u1ea3n \u1ee9ng trong k\u1ef9 thu\u1eadt Real-time PCR<\/p>\n<\/div>

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Probe (\u0111\u1ea7u d\u00f2) trong sinh h\u1ecdc ph\u00e2n t\u1eed l\u00e0 m\u1ed9t \u0111o\u1ea1n oligonucleotide (DNA ho\u1eb7c RNA s\u1ee3i \u0111\u01a1n) ng\u1eafn, \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng c\u00e1c ph\u00e2n t\u1eed ph\u00e1t t\u00edn hi\u1ec7u (th\u01b0\u1eddng l\u00e0 c\u00e1c ch\u1ea5t ph\u00e1t hu\u1ef3nh quang). Probe \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3…<\/p>\n<\/div>

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HPLC L\u00e0 G\u00ec? \u1ee8ng D\u1ee5ng C\u1ee7a HPLC Trong Tinh S\u1ea1ch v\u00e0 Ph\u00e2n T\u00edch Oligonucleotide<\/a><\/h3>
\n \n \n<\/svg>\nadmin<\/a><\/span>\n \n<\/svg>\nFeb 14, 2025<\/span>\n \n \n<\/svg>\n13 min read<\/span><\/div>

HPLC (High Performance Liquid Chromatography) \u2013 S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch, \u0111\u1ecbnh t\u00ednh ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng c\u00e1c ch\u1ea5t, th\u00e0nh ph\u1ea7n trong m\u1ed9t h\u1ed7n h\u1ee3p ch\u1ea5t l\u1ecfng.<\/p>\n<\/div>

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C\u00c1C PH\u01af\u01a0NG PH\u00c1P TINH S\u1ea0CH OLIGONUCLEOTIDE<\/a><\/h3>
\n \n \n<\/svg>\nadmin<\/a><\/span>\n \n<\/svg>\nJan 15, 2025<\/span>\n \n \n<\/svg>\n9 min read<\/span><\/div>

Oligonucleotide l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA m\u1ea1ch \u0111\u01a1n ho\u1eb7c m\u1ea1ch \u0111\u00f4i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed, y h\u1ecdc v\u00e0 nghi\u00ean c\u1ee9u khoa h\u1ecdc. C\u00e1c tr\u00ecnh t\u1ef1 oligonucleotide…<\/p>\n<\/div>

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D\u1ecbch v\u1ee5 OligoX<\/a><\/em><\/strong><\/h3>

T\u1ed5ng h\u1ee3p Oligonucleotide bao g\u1ed3m c\u00e1c s\u1ea3n ph\u1ea9m PCR, qPCR Primer, Primer\/Probe bi\u1ebfn \u0111\u1ed5i, Tr\u00ecnh t\u1ef1 RNA, Amplicon v\u00e0 c\u00e1c t\u1ed5ng h\u1ee3p \u0111\u1eb7c bi\u1ec7t<\/p><\/div>

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T\u00e0i li\u1ec7u tham kh\u1ea3o<\/h2>\n\n\n\n
    \n
  1. Click chemistry-based oligonucleotide labeling technology. (n.d.). https:\/\/www.lumiprobe.com\/probe-labeling-technology<\/a><\/li>\n\n\n\n
  2. Introduction to click chemistry: A new method for the labeling and modification of biomolecules. (n.d.). https:\/\/www.lumiprobe.com\/click-chemistry<\/a><\/li>\n\n\n\n
  3. Kaur, J., Saxena, M., & Rishi, N. (2021). An overview of recent advances in biomedical applications of click chemistry. Bioconjugate Chemistry, 32(8), 1455-1471.<\/li>\n\n\n\n
  4. The nobel prize in chemistry 2022. NobelPrize.org. (n.d.). https:\/\/www.nobelprize.org\/prizes\/chemistry\/2022\/popular-information\/<\/a><\/li>\n\n\n\n
  5. Fantoni, N. Z., El-Sagheer, A. H., & Brown, T. (2021). A hitchhiker\u2019s guide to click-chemistry with nucleic acids. Chemical Reviews, 121(12), 7122-7154.<\/li>\n\n\n\n
  6. Click chemistry and Nucleic Acids. ATDBio. (n.d.). https:\/\/atdbio.com\/nucleic-acids-book\/Click-chemistry-and-nucleic-acids<\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"

    Hi\u1ec7n nay, click chemistry \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn l\u00e0 m\u1ed9t t\u1eadp h\u1ee3p c\u00e1c ph\u1ea3n \u1ee9ng ch\u1ecdn l\u1ecdc, hi\u1ec7u qu\u1ea3 cao, ch\u00ednh x\u00e1c v\u00e0 nhanh ch\u00f3ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c h\u1ee3p ch\u1ea5t ti\u1ec1m n\u0103ng \u1ee9ng d\u1ee5ng trong c\u00e1c d\u01b0\u1ee3c ph\u1ea9m nh\u01b0 c\u00e1c ch\u1ea5t kh\u00e1ng virus, kh\u00e1ng khu\u1ea9n v\u00e0 \u1ee9ng d\u1ee5ng trong chu\u1ea9n \u0111o\u00e1n, h\u00ecnh \u1ea3nh y sinh.<\/p>","protected":false},"author":1,"featured_media":3263,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[28],"tags":[],"class_list":["post-3154","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-application"],"jetpack_featured_media_url":"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click.png","uagb_featured_image_src":{"full":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click.png",1920,1080,false],"thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-150x150.png",150,150,true],"medium":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-300x169.png",300,169,true],"medium_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-768x432.png",768,432,true],"large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-1024x576.png",1024,576,true],"1536x1536":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-1536x864.png",1536,864,true],"2048x2048":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click.png",1920,1080,false],"trp-custom-language-flag":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-18x10.png",18,10,true],"ultp_layout_landscape_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-1200x800.png",1200,800,true],"ultp_layout_landscape":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-870x570.png",870,570,true],"ultp_layout_portrait":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-600x900.png",600,900,true],"ultp_layout_square":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-600x600.png",600,600,true],"woocommerce_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-300x300.png",300,300,true],"woocommerce_single":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-600x338.png",600,338,true],"woocommerce_gallery_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2023\/12\/Danh-dau-oligo-va-dna-bang-ky-thuat-click-100x100.png",100,100,true]},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/oligox.abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":3,"uagb_excerpt":"Hi\u1ec7n nay, click chemistry \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn l\u00e0 m\u1ed9t t\u1eadp h\u1ee3p c\u00e1c ph\u1ea3n \u1ee9ng ch\u1ecdn l\u1ecdc, hi\u1ec7u qu\u1ea3 cao, ch\u00ednh x\u00e1c v\u00e0 nhanh ch\u00f3ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n c\u00e1c h\u1ee3p ch\u1ea5t ti\u1ec1m n\u0103ng \u1ee9ng d\u1ee5ng trong c\u00e1c d\u01b0\u1ee3c ph\u1ea9m nh\u01b0 c\u00e1c ch\u1ea5t kh\u00e1ng virus, kh\u00e1ng khu\u1ea9n v\u00e0 \u1ee9ng d\u1ee5ng trong chu\u1ea9n \u0111o\u00e1n, h\u00ecnh…","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3154","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=3154"}],"version-history":[{"count":7,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3154\/revisions"}],"predecessor-version":[{"id":3680,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3154\/revisions\/3680"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/media\/3263"}],"wp:attachment":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=3154"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=3154"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=3154"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}