{"id":3562,"date":"2025-01-15T08:06:40","date_gmt":"2025-01-15T08:06:40","guid":{"rendered":"https:\/\/oligox.abtvn.com\/?p=3562"},"modified":"2025-02-13T09:38:28","modified_gmt":"2025-02-13T09:38:28","slug":"phuong-phap-tinh-sach-oligonucleotide","status":"publish","type":"post","link":"https:\/\/oligox.abtvn.com\/en\/phuong-phap-tinh-sach-oligonucleotide\/","title":{"rendered":"C\u00c1C PH\u01af\u01a0NG PH\u00c1P TINH S\u1ea0CH OLIGONUCLEOTIDE"},"content":{"rendered":"<p class=\"wp-block-paragraph\"><a href=\"https:\/\/abtvn.com\/cac-chuoi-oligonucleotide-duoc-tong-hop-nhu-the-nao\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/cac-chuoi-oligonucleotide-duoc-tong-hop-nhu-the-nao\/\" target=\"_blank\" rel=\"noopener\"><strong>Oligonucleotide<\/strong> <\/a>l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA m\u1ea1ch \u0111\u01a1n ho\u1eb7c m\u1ea1ch \u0111\u00f4i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed, y h\u1ecdc v\u00e0 nghi\u00ean c\u1ee9u khoa h\u1ecdc. C\u00e1c tr\u00ecnh t\u1ef1 oligonucleotide th\u01b0\u1eddng \u0111\u01b0\u1ee3c t\u1ea1o ra d\u1ef1a tr\u00ean ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p h\u00f3a h\u1ecdc, trong \u0111\u00f3 c\u00e1c \u0111\u01a1n ph\u00e2n \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong ph\u01b0\u01a1ng ph\u00e1p h\u00f3a h\u1ecdc \u0111\u1ec3 c\u1ea5u th\u00e0nh m\u1ed9t tr\u00ecnh t\u1ef1 oligo, \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 phosphoramidite.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>T\u1ea1i sao ph\u1ea3i tinh s\u1ea1ch oligonucleotide<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Do b\u1ea3n ch\u1ea5t c\u1ee7a qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p oligonucleotide l\u00e0 c\u00e1c ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc m\u00e0 th\u00f4ng th\u01b0\u1eddng&nbsp; c\u00e1c ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc kh\u00f4ng bao gi\u1edd \u0111\u1ea1t \u0111\u01b0\u1ee3c hi\u1ec7u su\u1ea5t ph\u1ea3n \u1ee9ng l\u00e0 100%, do \u0111\u00f3 d\u1eabn \u0111\u1ebfn h\u00ecnh&nbsp; m\u1ed9t s\u1ed1 s\u1ea3n ph\u1ea9m ph\u1ee5 l\u1ed7i bao g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>C\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i, v\u00e0 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i n\u00e0y th\u01b0\u1eddng c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn h\u01a1n tr\u00ecnh t\u1ef1 oligonucleotide m\u1ee5c ti\u00eau c\u1ea7n t\u1ed5ng h\u1ee3p, do hi\u1ec7u su\u1ea5t ph\u1ea3n \u1ee9ng kh\u00f4ng \u0111\u1ea1t \u0111\u01b0\u1ee3c hi\u1ec7u qu\u1ea3 100% cho n\u00ean s\u1ebd c\u00f3 m\u1ed9t s\u1ed1 nucleotide kh\u00f4ng gh\u00e9p n\u1ed1i \u0111\u01b0\u1ee3c trong m\u1ed7i chu k\u1ef3 t\u1ed5ng h\u1ee3p t\u1eeb \u0111\u00f3 d\u1eabn \u0111\u1ebfn h\u00ecnh th\u00e0nh c\u00e1c tr\u00ecnh t\u1ef1 c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn h\u01a1n.<\/li>\n\n\n\n<li>C\u00e1c t\u1ea1p ch\u1ea5t h\u00f3a h\u1ecdc bao g\u1ed3m dung m\u00f4i, c\u00e1c ph\u00e2n t\u1eed nh\u1ecf nh\u01b0 c\u00e1c mu\u1ed1i h\u1eefu c\u01a1 \u0111\u01b0\u1ee3c t\u1ea1o th\u00e0nh t\u1eeb c\u00e1c ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc trong quy tr\u00ecnh t\u1ed5ng h\u1ee3p.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Vi\u1ec7c tinh ch\u1ebf gi\u00fap:<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li>\u0110\u1ea3m b\u1ea3o \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 c\u1ea3i thi\u1ec7n hi\u1ec7u qu\u1ea3 c\u1ee7a th\u00ed nghi\u1ec7m trong c\u00e1c \u1ee9ng d\u1ee5ng c\u1ee5 th\u1ec3 nh\u01b0 PCR, qPCR hay nghi\u00ean c\u1ee9u li\u1ec7u ph\u00e1p gene.<\/li>\n\n\n\n<li>\u0110\u1ea3m b\u1ea3o \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh v\u00e0 kh\u1ea3 n\u0103ng b\u1ea3o qu\u1ea3n c\u1ee7a s\u1ea3n ph\u1ea9m.<\/li>\n\n\n\n<li>\u0110\u00e1p \u1ee9ng c\u00e1c y\u00eau c\u1ea7u, ti\u00eau chu\u1ea9n nghi\u00eam ng\u1eb7t v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng trong c\u00e1c \u1ee9ng d\u1ee5ng l\u00e2m s\u00e0ng.<\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Ngu\u1ed3n g\u1ed1c c\u1ee7a c\u00e1c t\u1ea1p ch\u1ea5t v\u00e0 c\u00e1ch l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Trong qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t, m\u1ed7i nucleotide \u0111\u01b0\u1ee3c gh\u00e9p n\u1ed1i tu\u1ea7n t\u1ef1 v\u1edbi tr\u00ecnh t\u1ef1 \u0111ang t\u1ed5ng h\u1ee3p th\u00f4ng qua ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc phosphoramidite. Trong m\u1ed7i chu k\u1ef3 gh\u00e9p n\u1ed1i, c\u00f3 m\u1ed9t s\u1ed1 t\u1ef7 l\u1ec7 ph\u1ea7n tr\u0103m tr\u00ecnh t\u1ef1 oligo kh\u00f4ng \u0111\u01b0\u1ee3c gh\u00e9p n\u1ed1i v\u1edbi nucleotide ti\u1ebfp theo, n\u00ean t\u1eeb \u0111\u00f3 t\u1ea1o ra m\u1ed9t h\u1ed7n h\u1ee3p bao g\u1ed3m: c\u00e1c tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau ch\u00ednh x\u00e1c (n) v\u00e0 c\u00e1c tr\u00ecnh t\u1ef1 b\u1ecb \u0111\u1ed9t bi\u1ebfn c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn h\u01a1n (n-1, n-2, v.v.). Ngo\u00e0i ra, c\u00e1c t\u1ea1p ch\u1ea5t ph\u00e2n t\u1eed nh\u1ecf nh\u01b0 mu\u1ed1i h\u1eefu c\u01a1 l\u00e0 s\u1ea3n ph\u1ea9m ph\u1ee5 c\u1ee7a qu\u00e1 tr\u00ecnh c\u1eaft v\u00e0 kh\u1eed b\u1ea3o v\u1ec7. Vi\u1ec7c l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch n\u00ean c\u00e2n nh\u1eafc d\u1ef1a tr\u00ean c\u00e1c y\u1ebfu t\u1ed1 sau bao g\u1ed3m: chi\u1ec1u d\u00e0i oligo, lo\u1ea1i oligo v\u00e0 \u1ee9ng d\u1ee5ng c\u1ee7a oligo, chi ph\u00ed s\u1ea3n xu\u1ea5t v\u00e0 tinh s\u1ea1ch oligo. <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ed1i v\u1edbi c\u00e1c oligo ti\u00eau chu\u1ea9n c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn h\u01a1n nh\u1ecf h\u01a1n &lt; 35 bases v\u00e0 \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng trong c\u00e1c k\u1ef9 thu\u1eadt kh\u00f4ng c\u1ea7n \u0111\u1ed9 ch\u00ednh x\u00e1c cao nh\u01b0 PCR ho\u1eb7c RT-PCR th\u00ec c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng kh\u1eed mu\u1ed1i ho\u1eb7c catridge \u0111\u1ec3 tinh s\u1ea1ch. C\u00f2n \u0111\u1ed1i v\u1edbi oligo bi\u1ebfn \u0111\u1ed5i nh\u01b0 <a href=\"https:\/\/oligox.abtvn.com\/en\/reporter-va-quencher-cho-probe-qpcr\/\" data-type=\"link\" data-id=\"https:\/\/oligox.abtvn.com\/reporter-va-quencher-cho-probe-qpcr\/\">taqman probe<\/a> ho\u1eb7c c\u00e1c oligo \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng trong c\u00e1c nghi\u00ean c\u1ee9u y\u00eau c\u1ea7u \u0111\u1ed9 ch\u00ednh x\u00e1c cao nh\u01b0 t\u1ea1o d\u00f2ng (cloning), antisense hay trong t\u1ebf b\u00e0o s\u1ed1ng (in vivo) n\u00ean s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch HPLC. H\u00ecnh 1 cung c\u1ea5p m\u1ed9t s\u1ed1 khuy\u1ebfn ngh\u1ecb v\u1ec1 c\u00e1ch l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch t\u00f9y thu\u1ed9c v\u00e0o \u1ee9ng d\u1ee5ng c\u0169ng nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img fetchpriority=\"high\" decoding=\"async\" width=\"975\" height=\"639\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image.png\" alt=\"H\u00ecnh 1. B\u1ea3ng khuy\u1ebfn ngh\u1ecb ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch theo \u1ee9ng d\u1ee5ng nghi\u00ean c\u1ee9u trng t\u1ed5ng h\u1ee3p oligonucleotide\" class=\"wp-image-3563\" style=\"width:662px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image.png 975w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-300x197.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-768x503.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-18x12.png 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-870x570.png 870w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-600x393.png 600w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 1.<\/strong> B\u1ea3ng khuy\u1ebfn ngh\u1ecb ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch theo \u1ee9ng d\u1ee5ng nghi\u00ean c\u1ee9u<\/em><\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>C\u00e1c ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch oligo ph\u1ed5 bi\u1ebfn tr\u00ean th\u1ebf gi\u1edbi<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Ph\u01b0\u01a1ng ph\u00e1p kh\u1eed mu\u1ed1i<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Kh\u1eed mu\u1ed1i l\u00e0 ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch c\u01a1 b\u1ea3n ch\u1ec9 lo\u1ea1i b\u1ecf \u0111\u01b0\u1ee3c c\u00e1c t\u1ea1p ch\u1ea5t, c\u00e1c ph\u00e2n t\u1eed nh\u1ecf nh\u01b0 c\u00e1c mu\u1ed1i h\u1eefu c\u01a1 bao g\u1ed3m: benzamide, acrylonitrile \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh do c\u00e1c ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p oligo. H\u1ea7u h\u1ebft c\u00e1c s\u1ea3n p h\u1ea9m primer \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong k\u1ef9 thu\u1eadt PCR ho\u1eb7c qPCR th\u01b0\u1eddng \u0111\u01b0\u1ee3c tinh s\u1ea1ch b\u1eb1ng kh\u1eed mu\u1ed1i, b\u1edfi v\u00ec c\u00e1c primer th\u01b0\u1eddng c\u00f3 k\u00edch th\u01b0\u1edbc nh\u1ecf h\u01a1n &lt; 35 base, do \u0111\u00f3 t\u1ef7 l\u1ec7 tr\u00ecnh t\u1ef1 l\u1ed7i l\u00fac n\u00e0y ch\u1ec9 chi\u1ebfm m\u1ed9t t\u1ef7 l\u1ec7 \u00edt, \u0111\u1ed3ng th\u1eddi k\u1ef9 thu\u1eadt PCR kh\u00f4ng y\u00eau c\u1ea7u \u0111\u1ed9 ch\u00ednh x\u00e1c qu\u00e1 nghi\u00eam ng\u1eb7t khi so s\u00e1nh v\u1edbi c\u00e1c s\u1ea3n ph\u1ea9m oligo \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng tr\u00ean l\u00e2m s\u00e0ng nh\u01b0 antisense ho\u1eb7c gapmer. Do \u0111\u00f3 c\u00e1c oligo c\u00f3 chi\u1ec1u d\u00e0i nh\u1ecf h\u01a1n &lt; 35 bases c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p kh\u1eed mu\u1ed1i \u0111\u1ec3 tinh s\u1ea1ch. Nh\u01b0ng \u0111\u1ed1i v\u1edbi c\u00e1c oligo c\u00f3 k\u00edch th\u01b0\u1edbc &gt; 35 bases n\u00ean s\u1eed d\u1ee5ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch c\u00f3 kh\u1ea3 n\u0103ng lo\u1ea1i b\u1ecf \u0111\u01b0\u1ee3c c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p catridge ho\u1eb7c HPLC.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Hi\u1ec7n t\u1ea1i, k\u1ef9 thu\u1eadt s\u1eafc k\u00fd l\u1ecdc gel l\u00e0 m\u1ed9t trong nh\u1eefng k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t \u0111\u1ec3 tinh s\u1ea1ch kh\u1eed mu\u1ed1i cho c\u00e1c tr\u00ecnh t\u1ef1 oligo. Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt s\u1eafc k\u00fd l\u1ecdc gel l\u00e0 d\u1ef1a tr\u00ean s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 k\u00edch th\u01b0\u1edbc c\u1ee7a c\u00e1c ph\u00e2n t\u1eed c\u1ea7n ph\u00e2n t\u00e1ch. Trong \u0111\u00f3, c\u00e1c tr\u00ecnh t\u1ef1 oligo c\u1ea7n tinh s\u1ea1ch kh\u1eed mu\u1ed1i s\u1ebd \u0111\u01b0\u1ee3c n\u1ea1p v\u00e0o c\u00e1c c\u1ed9t c\u00f3 ch\u1ee9a c\u00e1c l\u1ed7 gel. Sau \u0111\u00f3 nh\u1eefng ph\u00e2n t\u1eed, t\u1ea1p ch\u1ea5t c\u00f3 k\u00edch th\u01b0\u1edbc nh\u1ecf s\u1ebd di chuy\u1ec3n xen k\u1ebd qua c\u00e1c l\u1ed7 gel n\u00ean di chuy\u1ec3n ch\u1eadm h\u01a1n, trong khi \u0111\u00f3 nh\u1eefng ph\u00e2n t\u1eed c\u00f3 k\u00edch th\u01b0\u1edbc l\u1edbn nh\u01b0 oligo kh\u00f4ng di chuy\u1ec3n \u0111\u01b0\u1ee3c qua c\u00e1c l\u1ed7 gel n\u00ean di chuy\u1ec3n nhanh h\u01a1n, d\u1eabn \u0111\u1ebfn c\u00e1c tr\u00ecnh t\u1ef1 oligo di chuy\u1ec3n ra kh\u1ecfi c\u1ed9t s\u1edbm h\u01a1n t\u1eeb \u0111\u00f3 ph\u00e2n t\u00e1ch \u0111\u01b0\u1ee3c c\u00e1c t\u1ea1p ch\u1ea5t c\u00f3 k\u00edch th\u01b0\u1edbc nh\u1ecf nh\u01b0 mu\u1ed1i.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img decoding=\"async\" width=\"860\" height=\"669\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1.jpg\" alt=\"\" class=\"wp-image-3564\" style=\"width:461px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1.jpg 860w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-300x233.jpg 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-768x597.jpg 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-15x12.jpg 15w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-600x467.jpg 600w\" sizes=\"(max-width: 860px) 100vw, 860px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 2.<\/strong> Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt s\u1eafc k\u00fd l\u1ecdc gel (s\u1eafc k\u00fd r\u00e2y ph\u00e2n t\u1eed)<\/em><\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Ph\u01b0\u01a1ng ph\u00e1p reversed-phase cartridge<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">C\u00e1c tr\u00ecnh t\u1ef1 \u0111\u01b0\u1ee3c tinh s\u1ea1ch b\u1eb1ng k\u1ef9 thu\u1eadt catridge pha \u0111\u1ea3o c\u00f3 \u0111\u1ed9 tinh s\u1ea1ch cao h\u01a1n so v\u1edbi ph\u01b0\u01a1ng ph\u00e1p kh\u1eed mu\u1ed1i, v\u00ec k\u1ef9 thu\u1eadt n\u00e0y c\u00f3 kh\u1ea3 n\u0103ng lo\u1ea1i b\u1ecf \u0111\u01b0\u1ee3c c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i. Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt n\u00e0y l\u00e0 d\u1ef1a tr\u00ean s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 t\u00ednh k\u1ef5 n\u01b0\u1edbc gi\u1eefa tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau c\u00f3 k\u00edch th\u01b0\u1edbc ch\u00ednh x\u00e1c (th\u01b0\u1eddng \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c nh\u00f3m 5\u2019-DMT k\u1ef5 n\u01b0\u1edbc), trong khi \u0111\u00f3 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn h\u01a1n th\u01b0\u1eddng s\u1ebd kh\u00f4ng \u0111\u01b0\u1ee3c g\u1eafn c\u00e1c nh\u00f3m 5\u2019-DMT.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">C\u00e1c m\u1eabu oligo c\u1ea7n tinh s\u1ea1ch s\u1ebd \u0111\u01b0\u1ee3c n\u1ea1p v\u00e0o c\u1ed9t c\u00f3 ch\u1ee9a c\u00e1c pha t\u0129nh mang nh\u00f3m ch\u1ee9c k\u1ef5 n\u01b0\u1edbc, l\u00fac n\u00e0y c\u00e1c tr\u00ecnh t\u1ef1 c\u00f3 k\u00edch th\u01b0\u1edbc \u0111\u1ea7y \u0111\u1ee7 v\u00e0 c\u00f3 ch\u1ee9a nh\u00f3m 5\u2019-DMT k\u1ef5 n\u01b0\u1edbc (\u0110o\u1ea1n th\u1eb3ng c\u00f3 g\u1eafn ng\u00f4i sao nh\u01b0 h\u00ecnh 3) th\u01b0\u1eddng c\u00f3 li\u00ean k\u1ebft m\u1ea1nh v\u1edbi c\u1ed9t n\u00ean c\u00e1c tr\u00ecnh t\u1ef1 oligo c\u00f3 k\u00edch th\u01b0\u1edbc ch\u00ednh x\u00e1c s\u1ebd \u0111\u01b0\u1ee3c gi\u1eef l\u1ea1i tr\u00ean c\u1ed9t, trong khi \u0111\u00f3 nh\u1eefng tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn s\u1ebd b\u1ecb r\u1eeda gi\u1ea3i s\u1edbm h\u01a1n. Sau \u0111\u00f3 s\u1ebd ti\u1ebfn h\u00e0nh lo\u1ea1i b\u1ecf nh\u00f3m DMT v\u00e0 r\u1ed3i ti\u1ebfn h\u00e0nh thu nh\u1eadn tr\u00ecnh t\u1ef1 oligo m\u1ee5c ti\u00eau.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img decoding=\"async\" width=\"860\" height=\"683\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-1.jpg\" alt=\"\" class=\"wp-image-3565\" style=\"width:492px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-1.jpg 860w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-1-300x238.jpg 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-1-768x610.jpg 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-1-15x12.jpg 15w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-1-600x477.jpg 600w\" sizes=\"(max-width: 860px) 100vw, 860px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 3.<\/strong> Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt catridge pha \u0111\u1ea3o<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">Khi chi\u1ec1u d\u00e0i oligonucleotide t\u0103ng l\u00ean, t\u1ef7 l\u1ec7 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 xu h\u01b0\u1edbng t\u0103ng l\u00ean v\u00e0 \u0111\u01b0\u1ee3c gi\u1eef l\u1ea1i tr\u00ean c\u1ed9t. Nh\u1eefng tr\u00ecnh t\u1ef1 l\u1ed7i kh\u00f4ng mong mu\u1ed1n n\u00e0y s\u1ebd kh\u00f4ng \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf b\u1eb1ng k\u1ef9 thu\u1eadt catridge v\u00e0 do \u0111\u00f3, \u0111\u1ed1i v\u1edbi c\u00e1c oligonucleotide d\u00e0i h\u01a1n &gt; 60 bases, n\u00ean s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao (HPLC) ho\u1eb7c \u0111i\u1ec7n di gel polyacrylamide (PAGE).<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao pha \u0111\u1ea3o (RP-HPLC)<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao pha \u0111\u1ea3o (RP-HPLC)<\/strong> ho\u1ea1t \u0111\u1ed9ng theo c\u00f9ng nguy\u00ean l\u00fd nh\u01b0 k\u1ef9 thu\u1eadt catridge pha \u0111\u1ea3o. Tuy nhi\u00ean, RP-HPLC c\u00f3 kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch t\u1ed1t h\u01a1n d\u1eabn \u0111\u1ebfn c\u00e1c s\u1ea3n ph\u1ea9m c\u00f3 m\u1ee9c \u0111\u1ed9 tinh khi\u1ebft cao h\u01a1n. RP-HPLC l\u00e0 ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch hi\u1ec7u qu\u1ea3 \u0111\u1ed1i v\u1edbi c\u00e1c oligonucleotide bi\u1ebfn \u0111\u1ed5i c\u00f3 g\u1eafn hu\u1ef3nh quang v\u00ec t\u00ednh k\u1ef5 n\u01b0\u1edbc c\u1ee7a hu\u1ef3nh quang gi\u00fap ph\u00e2n t\u00e1ch hi\u1ec7u qu\u1ea3 tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau kh\u1ecfi c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn v\u00e0 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i kh\u00f4ng g\u1eafn hu\u1ef3nh quang. H\u01a1n n\u1eefa, RP-HPLC l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn \u0111\u1ec3 t\u1ed5ng h\u1ee3p quy m\u00f4 l\u1edbn do kh\u1ea3 n\u0103ng t\u1ef1 \u0111\u1ed9ng h\u00f3a c\u1ee7a h\u1ec7 th\u1ed1ng.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Tuy nhi\u00ean, kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch d\u1ef1a tr\u00ean t\u00ednh k\u1ef5 n\u01b0\u1edbc c\u00f3 th\u1ec3 b\u1ecb \u1ea3nh h\u01b0\u1edfng theo chi\u1ec1u d\u00e0i c\u1ee7a oligonucleotide. Do \u0111\u00f3, RP-HPLC th\u01b0\u1eddng kh\u00f4ng \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb \u0111\u1ec3 tinh ch\u1ebf oligonucleotide &gt;60 bases. M\u1eb7c d\u00f9 c\u00f3 th\u1ec3 tinh ch\u1ebf oligonucleotide d\u00e0i h\u01a1n (l\u00ean \u0111\u1ebfn 80 bases, trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p d\u00e0i h\u01a1n) b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, nh\u01b0ng \u0111\u1ed9 tinh khi\u1ebft v\u00e0 n\u0103ng su\u1ea5t c\u00f3 th\u1ec3 b\u1ecb \u1ea3nh h\u01b0\u1edfng x\u1ea5u. C\u00e1c s\u1ea3n ph\u1ea9m oligo sau khi tinh s\u1ea1ch b\u1eb1ng RP-HPLC th\u01b0\u1eddng c\u00f3 \u0111\u1ed9 tinh khi\u1ebft &gt;90% (th\u00f4ng qua ph\u00e2n t\u00edch HPLC).<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u su\u1ea5t cao trao \u0111\u1ed5i ion (IE-HPLC)<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u su\u1ea5t cao trao \u0111\u1ed5i ion (IE-HPLC) <\/strong>(c\u1ee5 th\u1ec3 l\u00e0 trao \u0111\u1ed5i anion) d\u1ef1a tr\u00ean s\u1ed1 l\u01b0\u1ee3ng nh\u00f3m t\u00edch \u0111i\u1ec7n \u00e2m c\u1ee7a nh\u00f3m phosphate trong khung x\u01b0\u01a1ng ch\u00ednh c\u1ee7a oligonucleotide. Ph\u01b0\u01a1ng ph\u00e1p trao \u0111\u1ed5i anion li\u00ean quan \u0111\u1ebfn vi\u1ec7c s\u1eed d\u1ee5ng c\u00e1c pha \u0111\u1ed9ng c\u00f3 n\u1ed3ng \u0111\u1ed9 mu\u1ed1i cao nh\u01b0 Nacl tr\u00ean c\u00e1c c\u1ed9t c\u00f3 ch\u1ee9a c\u00e1c pha t\u0129nh amoni. C\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn (shortmer) th\u01b0\u1eddng c\u00f3 s\u1ed1 l\u01b0\u1ee3ng \u0111i\u1ec7n t\u00edch \u00e2m \u00edt h\u01a1n so v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau do \u0111\u00f3 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i th\u01b0\u1eddng c\u00f3 li\u00ean k\u1ebft y\u1ebfu v\u1edbi c\u1ed9t n\u00ean \u0111\u01b0\u1ee3c r\u1eeda gi\u1ea3i s\u1edbm h\u01a1n so v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau (h\u00ecnh 4). Tuy nhi\u00ean, IE-HPLC b\u1ecb gi\u1edbi h\u1ea1n b\u1edfi \u0111\u1ed9 d\u00e0i, th\u01b0\u1eddng kho\u1ea3ng 40 bases. C\u00e1c oligonucleotide d\u00e0i h\u01a1n d\u1eabn \u0111\u1ebfn kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch th\u1ea5p h\u01a1n gi\u1eefa tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau v\u00e0 tr\u00ecnh t\u1ef1 l\u1ed7i; v\u00e0 do \u0111\u00f3, \u0111\u1ed9 tinh khi\u1ebft th\u01b0\u1eddng th\u1ea5p h\u01a1n v\u00e0 k\u00e9m nh\u1ea5t qu\u00e1n h\u01a1n.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">L\u00fd do ch\u00ednh \u0111\u1ec3 s\u1eed d\u1ee5ng IE-HPLC thay v\u00ec RP-HPLC l\u00e0 \u0111\u1ec3 tinh ch\u1ebf c\u00e1c oligonucleotide c\u00f3 mang c\u00e1c c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p ph\u1ee9c t\u1ea1p, th\u01b0\u1eddng \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y trong c\u00e1c tr\u00ecnh t\u1ef1 c\u00f3 h\u00e0m l\u01b0\u1ee3ng GC cao. IE-HPLC c\u00f3 hi\u1ec7u qu\u1ea3 \u0111\u1ed1i v\u1edbi c\u00e1c oligonucleotide nh\u01b0 v\u1eady v\u00ec pha \u0111\u1ed9ng c\u00f3 \u0111\u1ed9 pH cao, l\u00e0m \u0111\u1ee9t g\u00e3y c\u00e1c li\u00ean k\u1ebft hydro v\u00e0 do \u0111\u00f3, ph\u00e1 v\u1ee1 c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p. K\u1ef9 thu\u1eadt IE-HPLC n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n sau qu\u00e1 tr\u00ecnh tinh s\u1ea1ch RP-HPLC \u0111\u1ec3 t\u0103ng \u0111\u1ed9 tinh khi\u1ebft c\u1ee7a s\u1ea3n ph\u1ea9m oligo.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"860\" height=\"601\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-2.jpg\" alt=\"\" class=\"wp-image-3566\" style=\"width:534px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-2.jpg 860w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-2-300x210.jpg 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-2-768x537.jpg 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-2-18x12.jpg 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-2-600x419.jpg 600w\" sizes=\"(max-width: 860px) 100vw, 860px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 4. <\/strong>Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt tinh s\u1ea1ch IE-HPLC<\/em><\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec7n di gel polyacrylamide (PAGE)<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u0110i\u1ec7n di gel polyacrylamide (PAGE)<\/strong> s\u1eed d\u1ee5ng m\u00f4i tr\u01b0\u1eddng bi\u1ebfn t\u00ednh c\u1ee5 th\u1ec3 l\u00e0 m\u00f4i tr\u01b0\u1eddng c\u00f3 ch\u1ee9a URE (URE PAGE) \u0111\u1ec3 ph\u00e2n c\u00e1c oligonucleotide d\u1ef1a tr\u00ean tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed thay v\u00ec \u0111i\u1ec7n t\u00edch. Trong \u0111i\u1ec1u ki\u1ec7n th\u00edch h\u1ee3p, c\u00e1c oligonucleotide kh\u00e1c nhau b\u1edfi 1 nucleotide duy nh\u1ea5t c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch. Kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch k\u00edch th\u01b0\u1edbc hi\u1ec7u qu\u1ea3 n\u00e0y th\u01b0\u1eddng mang l\u1ea1i m\u1ee9c \u0111\u1ed9 tinh khi\u1ebft cao nh\u1ea5t c\u00f3 th\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c trong b\u1ea5t k\u1ef3 ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch n\u00e0o hi\u1ec7n c\u00f3. Hi\u1ec7u su\u1ea5t thu h\u1ed3i m\u1ee5c ti\u00eau t\u1eeb k\u1ef9 thu\u1eadt PAGE th\u01b0\u1eddng th\u1ea5p h\u01a1n so v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c do kh\u1ea3 n\u0103ng lo\u1ea1i b\u1ecf tr\u00ecnh t\u1ef1 l\u1ed7i v\u00e0 c\u0169ng nh\u01b0 quy tr\u00ecnh ph\u1ee9c t\u1ea1p \u0111\u1ec3 ph\u00e2n t\u00e1ch \u0111\u01b0\u1ee3c c\u00e1c oligonucleotide m\u1ee5c ti\u00eau. PAGE th\u01b0\u1eddng \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb khi c\u1ea7n c\u00e1c s\u1ea3n ph\u1ea9m c\u00f3 \u0111\u1ed9 tinh khi\u1ebft cao \u0111\u1ec3 \u1ee9ng d\u1ee5ng trong c\u00e1c th\u00ed nghi\u1ec7m y\u00eau c\u1ea7u \u0111\u1ed9 ch\u00ednh x\u00e1c cao v\u00e0 \u0111\u1ed1i v\u1edbi c\u00e1c tr\u00ecnh t\u1ef1 c\u00f3 chi\u1ec1u d\u00e0i l\u1edbn h\u01a1n \u2265 60 baz\u01a1. C\u00e1c s\u1ea3n ph\u1ea9m sau khi tinh s\u1ea1ch b\u1eb1ng PAGE th\u01b0\u1eddng c\u00f3 \u0111\u1ed9 tinh khi\u1ebft cao &gt;95%.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"860\" height=\"656\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1.png\" alt=\"\" class=\"wp-image-3567\" style=\"width:519px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1.png 860w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-300x229.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-768x586.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-16x12.png 16w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/image-1-600x458.png 600w\" sizes=\"(max-width: 860px) 100vw, 860px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 5.<\/strong> Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt tinh s\u1ea1ch PAGE<\/em><\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>T\u00c0I LI\u1ec6U THAM KH\u1ea2O<\/strong><\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li><em>Oligonucleotide purification methods for your research &#8211; UK<\/em>. (n.d.). Retrieved January 8, 2025, from <a href=\"https:\/\/www.thermofisher.com\/vn\/en\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/spotlight-articles\/oligonucleotide-purification-methods.html\" target=\"_blank\" rel=\"noopener\">https:\/\/www.thermofisher.com\/vn\/en\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/spotlight-articles\/oligonucleotide-purification-methods.html<\/a><\/li>\n\n\n\n<li>(N.d.). Sigmaaldrich.com. Retrieved January 8, 2025, from https:\/\/www.sigmaaldrich.com\/VN\/en\/technical-documents\/technical-article\/genomics\/dna-and-rna-purification\/best-purification<\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>Oligonucleotide l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA m\u1ea1ch \u0111\u01a1n ho\u1eb7c m\u1ea1ch \u0111\u00f4i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed, y h\u1ecdc v\u00e0 nghi\u00ean c\u1ee9u khoa h\u1ecdc. C\u00e1c tr\u00ecnh t\u1ef1 oligonucleotide th\u01b0\u1eddng \u0111\u01b0\u1ee3c t\u1ea1o ra d\u1ef1a tr\u00ean ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p h\u00f3a h\u1ecdc, trong \u0111\u00f3 c\u00e1c &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"\" href=\"https:\/\/oligox.abtvn.com\/en\/phuong-phap-tinh-sach-oligonucleotide\/\"> <span class=\"screen-reader-text\">C\u00c1C PH\u01af\u01a0NG PH\u00c1P TINH S\u1ea0CH OLIGONUCLEOTIDE<\/span> Read More \u00bb<\/a><\/p>","protected":false},"author":1,"featured_media":3570,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[28,23],"tags":[],"class_list":["post-3562","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-application","category-bai-viet"],"jetpack_featured_media_url":"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2.jpg","uagb_featured_image_src":{"full":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2.jpg",1920,1080,false],"thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-150x150.jpg",150,150,true],"medium":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-300x169.jpg",300,169,true],"medium_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-768x432.jpg",768,432,true],"large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-1024x576.jpg",1024,576,true],"1536x1536":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-1536x864.jpg",1536,864,true],"2048x2048":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2.jpg",1920,1080,false],"trp-custom-language-flag":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-18x10.jpg",18,10,true],"ultp_layout_landscape_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-1200x800.jpg",1200,800,true],"ultp_layout_landscape":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-870x570.jpg",870,570,true],"ultp_layout_portrait":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-600x900.jpg",600,900,true],"ultp_layout_square":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-600x600.jpg",600,600,true],"woocommerce_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-300x300.jpg",300,300,true],"woocommerce_single":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-600x338.jpg",600,338,true],"woocommerce_gallery_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/01\/Banner-blog-website-2-100x100.jpg",100,100,true]},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/oligox.abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":3,"uagb_excerpt":"Oligonucleotide l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA m\u1ea1ch \u0111\u01a1n ho\u1eb7c m\u1ea1ch \u0111\u00f4i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed, y h\u1ecdc v\u00e0 nghi\u00ean c\u1ee9u khoa h\u1ecdc. C\u00e1c tr\u00ecnh t\u1ef1 oligonucleotide th\u01b0\u1eddng \u0111\u01b0\u1ee3c t\u1ea1o ra d\u1ef1a tr\u00ean ph\u01b0\u01a1ng ph\u00e1p t\u1ed5ng h\u1ee3p h\u00f3a h\u1ecdc, trong \u0111\u00f3 c\u00e1c&hellip;","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3562","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=3562"}],"version-history":[{"count":4,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3562\/revisions"}],"predecessor-version":[{"id":3576,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3562\/revisions\/3576"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/media\/3570"}],"wp:attachment":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=3562"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=3562"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=3562"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}