{"id":3578,"date":"2025-02-14T06:16:00","date_gmt":"2025-02-14T06:16:00","guid":{"rendered":"https:\/\/oligox.abtvn.com\/?p=3578"},"modified":"2025-08-01T04:12:20","modified_gmt":"2025-08-01T04:12:20","slug":"ung-dung-cua-hplc-trong-tinh-sach-oligo","status":"publish","type":"post","link":"https:\/\/oligox.abtvn.com\/en\/ung-dung-cua-hplc-trong-tinh-sach-oligo\/","title":{"rendered":"HPLC L\u00e0 G\u00ec? \u1ee8ng D\u1ee5ng C\u1ee7a HPLC Trong Tinh S\u1ea1ch v\u00e0 Ph\u00e2n T\u00edch Oligonucleotide"},"content":{"rendered":"<h2 class=\"wp-block-heading\"><strong>1. T\u1ed5ng quan v\u1ec1 HPLC<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><a href=\"https:\/\/oligox.abtvn.com\/en\/phuong-phap-tinh-sach-oligonucleotide\/\" data-type=\"link\" data-id=\"https:\/\/oligox.abtvn.com\/phuong-phap-tinh-sach-oligonucleotide\/\">HPLC (High Performance Liquid Chromatography)<\/a><\/strong> \u2013 <strong>S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao<\/strong>. \u0110\u00e2y l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch, \u0111\u1ecbnh t\u00ednh ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng c\u00e1c ch\u1ea5t, th\u00e0nh ph\u1ea7n trong m\u1ed9t h\u1ed7n h\u1ee3p ch\u1ea5t l\u1ecfng.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>2. L\u1ecbch s\u1eed c\u1ee7a HPLC<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">B\u1ea3n ch\u1ea5t c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p HPLC l\u00e0 ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd c\u1ed5 \u0111i\u1ec3n nh\u01b0ng \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch d\u01b0\u1edbi \u0111i\u1ec1u ki\u1ec7n \u00e1p su\u1ea5t cao v\u1edbi s\u1ef1 h\u1ed7 tr\u1ee3 c\u1ee7a c\u00e1c c\u00e1c m\u00e1y b\u01a1m cao \u00e1p. Ngu\u1ed3n g\u1ed1c c\u1ee7a t\u1eeb vi\u1ebft t\u1eaft HPLC, \u0111\u01b0\u1ee3c gi\u1edbi thi\u1ec7u l\u1ea7n \u0111\u1ea7u ti\u00ean b\u1edfi Gi\u00e1o s\u01b0 Csaba Horv\u00e1th trong b\u00e0i b\u00e1o Pittcon n\u0103m 1970, \u00e1p su\u1ea5t cao l\u00fac n\u00e0y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u1ea1o ra d\u00f2ng ch\u1ea3y qua c\u1ed9t s\u1eafc k\u00fd. L\u00fac n\u00e0y c\u00e1c m\u00e1y b\u01a1m ch\u1ec9 c\u00f3 kh\u1ea3 n\u0103ng t\u1ea1o ra \u00e1p su\u1ea5t 500 psi t\u01b0\u01a1ng \u0111\u01b0\u01a1ng v\u1edbi 35 bar. \u1ede th\u1eddi \u0111i\u1ec3m n\u00e0y \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 s\u1eafc k\u00fd l\u1ecfng \u00e1p su\u1ea5t cao (high pressure liquid chromatography) . \u0110\u1ea7u nh\u1eefng n\u0103m 1970 ch\u1ee9ng ki\u1ebfn \u200b\u200bb\u01b0\u1edbc nh\u1ea3y v\u1ecdt v\u1ec1 c\u00f4ng ngh\u1ec7, c\u00e1c thi\u1ebft b\u1ecb HPLC m\u1edbi \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n c\u00f3 \u00e1p su\u1ea5t l\u00ean t\u1edbi 6.000 psi (400 bar) v\u00e0 k\u1ebft h\u1ee3p c\u00e1c kim ti\u00eam m\u1eabu, \u0111\u1ea7u d\u00f2 v\u00e0 c\u1ed9t \u0111\u01b0\u1ee3c c\u1ea3i ti\u1ebfn. . V\u1edbi nh\u1eefng ti\u1ebfn b\u1ed9 li\u00ean t\u1ee5c v\u1ec1 hi\u1ec7u su\u1ea5t trong th\u1eddi gian n\u00e0y \u00a0bao g\u1ed3m: c\u00e1c h\u1ea1t nh\u1ecf h\u01a1n, \u00e1p su\u1ea5t cao h\u01a1n d\u1eabn \u0111\u1ebfn t\u00ean c\u1ee7a k\u1ef9 thu\u1eadt n\u00e0y \u0111\u00e3 \u0111\u1ed5i th\u00e0nh s\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao (high performance liquid chromatography) nh\u01b0ng t\u1eeb vi\u1ebft t\u1eaft HPLC v\u1eabn gi\u1eef nguy\u00ean.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>3. C\u1ea5u t\u1ea1o HPLC<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">M\u1ed9t h\u1ec7 th\u1ed1ng HPLC th\u01b0\u1eddng bao g\u1ed3m nhi\u1ec1u b\u1ed9 ph\u1eadn kh\u00e1c nhau, trong \u0111\u00f3 c\u00e1c b\u1ed9 ph\u1eadn ch\u00ednh th\u01b0\u1eddng bao g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>B\u00ecnh ch\u1ee9a pha \u0111\u1ed9ng (Solvent Reservoir):\u00a0 <\/strong>m\u1ed9t m\u00e1y HPLC th\u00f4ng th\u01b0\u1eddng c\u00f3 t\u1eeb 2 \u0111\u1ebfn 4 b\u00ecnh ch\u1ee9a pha \u0111\u1ed9ng \u0111\u1ec3 tr\u1ed9n c\u00e1c dung m\u00f4i kh\u00e1c nhau \u0111\u1ec3 ph\u00e2n t\u00e1ch v\u00e0 r\u1eeda gi\u1ea3i theo t\u1ef7 l\u1ec7 mong mu\u1ed1n.<\/li>\n\n\n\n<li><strong>B\u1ed9 kh\u1eed kh\u00ed (Degasser):<\/strong> c\u00f3 ch\u1ee9c n\u0103ng h\u1ed7 tr\u1ee3 lo\u1ea1i b\u1ecf nh\u1eefng b\u1ecdt kh\u00ed c\u00f2n s\u00f3t l\u1ea1i trong dung m\u00f4i pha \u0111\u1ed9ng \u0111\u1ec3 gi\u00fap b\u1ed9 ph\u1eadn b\u01a1m ho\u1ea1t \u0111\u1ed9ng \u1ed5n \u0111\u1ecbnh v\u00e0 ch\u00ednh x\u00e1c, do khi b\u1ecdt kh\u00ed qu\u00e1 nhi\u1ec1u kh\u00f4ng th\u1ec3 lo\u1ea1i tr\u1eeb h\u1ebft ch\u00fang th\u00ec b\u1ed9 ph\u1eadn b\u01a1m cao \u00e1p c\u00f3 th\u1ec3 kh\u00f4ng h\u00fat \u0111\u01b0\u1ee3c dung m\u00f4i, \u1ea3nh h\u01b0\u1edfng t\u1edbi t\u1ef7 l\u1ec7 ph\u1ed1i tr\u1ed9n gi\u1eefa c\u00e1c dung m\u00f4i v\u00e0 t\u1eeb \u0111\u00f3 d\u1eabn \u0111\u1ebfn sai l\u1ec7ch k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch cu\u1ed1i c\u00f9ng c\u1ee7a h\u1ec7 th\u1ed1ng.<\/li>\n\n\n\n<li><strong>B\u01a1m cao \u00e1p (High-Pressure Pump):<\/strong> c\u00f3 ch\u1ee9c n\u0103ng b\u01a1m pha \u0111\u1ed9ng \u0111i qua h\u1ec7 th\u1ed1ng v\u1edbi \u00e1p su\u1ea5t cao \u0111\u1ec3 th\u1ef1c hi\u1ec7n qu\u00e1 tr\u00ecnh ph\u00e2n t\u00e1ch s\u1eafc k\u00fd, th\u01b0\u1eddng \u0111\u1ea1t \u00e1p su\u1ea5t kho\u1ea3ng 1000\u20135000 psi t\u00f9y thu\u1ed9c v\u00e0o t\u1ed1c \u0111\u1ed9 d\u00f2ng s\u1eed d\u1ee5ng.<\/li>\n\n\n\n<li><strong>B\u1ed9 ti\u00eam m\u1eabu (Injector ho\u1eb7c Autosa mpler):<\/strong> c\u00f3 ch\u1ee9c n\u0103ng ti\u00eam m\u1eabu c\u1ea7n ph\u00e2n t\u00edch v\u00e0o c\u1ed9t ph\u00e2n t\u00edch.<\/li>\n\n\n\n<li><strong>C\u1ed9t s\u1eafc k\u00fd (Column):<\/strong> L\u00e0 n\u01a1i di\u1ec5n ra qu\u00e1 tr\u00ecnh ph\u00e2n t\u00e1ch s\u1eafc k\u00fd v\u00e0 th\u01b0\u1eddng \u0111\u01b0\u1ee3c gi\u1eef \u1edf nhi\u1ec7t \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh trong bu\u1ed3ng \u0111i\u1ec1u nhi\u1ec7t c\u1ed9t. C\u1ed9t s\u1eafc k\u00fd \u0111\u01b0\u1ee3c l\u00e0m t\u1eeb th\u00e9p kh\u00f4ng g\u1ec9 v\u1edbi chi\u1ec1u d\u00e0i kho\u1ea3ng 50 \u2013 300 mm v\u1edbi \u0111\u01b0\u1eddng k\u00ednh trong t\u1eeb 2 \u2013 5 mm v\u00e0 th\u01b0\u1eddng \u0111\u01b0\u1ee3c nh\u1ed3i b\u1eb1ng c\u00e1c h\u1ea1t silica nh\u1ecf c\u00f3 k\u00edch th\u01b0\u1edbc t\u1eeb 2.5 \u2013 5 \u00b5m \u0111\u01b0\u1ee3c bi\u1ebfn \u0111\u1ed5i \u0111\u1ec3 ph\u00f9 h\u1ee3p v\u1edbi lo\u1ea1i ph\u01b0\u01a1ng ph\u00e1p HPLC (pha \u0111\u1ea3o, pha th\u01b0\u1eddng, trao \u0111\u1ed5i ion)<\/li>\n\n\n\n<li><strong>\u0110\u1ea7u d\u00f2 (Detector):<\/strong> c\u00f3 ch\u1ee9c n\u0103ng ph\u00e1t hi\u1ec7n v\u00e0 ghi nh\u1eadn t\u00edn hi\u1ec7u c\u1ee7a c\u00e1c h\u1ee3p ch\u1ea5t khi ch\u00fang ra kh\u1ecfi c\u1ed9t s\u1eafc k\u00fd \u0111\u1ec3 \u0111\u1ecbnh t\u00ednh v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng. T\u00f9y thu\u1ed9c v\u00e0o t\u00ednh ch\u1ea5t c\u1ee7a ch\u1ea5t ph\u00e2n t\u00edch m\u00e0 ng\u01b0\u1eddi ta ch\u1ecdn lo\u1ea1i \u0111\u1ea7u d\u00f2 ph\u00f9 h\u1ee3p. C\u00e1c lo\u1ea1i \u0111\u1ea7u d\u00f2 ph\u1ed5 bi\u1ebfn th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng bao g\u1ed3m: UV-VIS, PDA, RI, EC, FL.<\/li>\n\n\n\n<li><strong>B\u1ed9 x\u1eed l\u00fd d\u1eef li\u1ec7u (Data System\/Software): <\/strong>c\u00f3 ch\u1ee9c n\u0103ng thu th\u1eadp v\u00e0 x\u1eed l\u00fd t\u00edn hi\u1ec7u t\u1eeb \u0111\u1ea7u d\u00f2, t\u1ea1o s\u1eafc k\u00fd \u0111\u1ed3 (chromatogram) \u0111\u1ec3 ph\u00e2n t\u00edch, ghi nh\u1eadn th\u1eddi gian l\u01b0u (retention time). ,\u0111o di\u1ec7n t\u00edch ho\u1eb7c chi\u1ec1u cao c\u00e1c \u0111\u1ec9nh (peaks) \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img fetchpriority=\"high\" decoding=\"async\" width=\"806\" height=\"750\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image.png\" alt=\"H\u00ecnh. C\u1ea5u t\u1ea1o h\u1ec7 th\u1ed1ng HPLC \" class=\"wp-image-3579\" style=\"width:699px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image.png 806w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-300x279.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-768x715.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-13x12.png 13w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-600x558.png 600w\" sizes=\"(max-width: 806px) 100vw, 806px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 1. <\/strong>C\u1ea5u t\u1ea1o h\u1ec7 th\u1ed1ng HPLC<\/em><\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>4. Nguy\u00ean l\u00fd ho\u1ea1t \u0111\u1ed9ng c\u1ee7a HPLC<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Qu\u00e1 tr\u00ecnh ho\u1ea1t \u0111\u1ed9ng, ph\u00e2n t\u00e1ch h\u1ed7n h\u1ee3p ch\u1ea5t c\u1ee7a HPLC th\u01b0\u1eddng d\u1ef1a tr\u00ean s\u1ef1 t\u01b0\u01a1ng t\u00e1c, s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 \u00e1i l\u1ef1c c\u1ee7a h\u1ed7n h\u1ee3p ch\u1ea5t c\u1ea7n ph\u00e2n t\u00e1ch v\u1edbi pha \u0111\u1ed9ng trong dung m\u00f4i v\u00e0 pha t\u0129nh trong c\u1ed9t s\u1eafc k\u00fd. Khi h\u1ed7n h\u1ee3p m\u1eabu \u0111\u01b0\u1ee3c ti\u00eam v\u00e0o h\u1ec7 th\u1ed1ng, h\u1ed7n h\u1ee3p ch\u1ea5t c\u1ea7n ph\u00e2n t\u00e1ch s\u1ebd di chuy\u1ec3n qua c\u1ed9t v\u1edbi t\u1ed1c \u0111\u1ed9 kh\u00e1c nhau t\u00f9y theo m\u1ee9c \u0111\u1ed9 t\u01b0\u01a1ng t\u00e1c, \u00e1i l\u1ef1c c\u1ee7a m\u1ed7i ch\u1ea5t v\u1edbi pha t\u0129nh v\u00e0 pha \u0111\u1ed9ng. C\u00e1c ch\u1ea5t, ph\u00e2n t\u1eed c\u00f3 \u00e1i l\u1ef1c m\u1ea1nh v\u1edbi pha t\u0129nh s\u1ebd li\u00ean k\u1ebft m\u1ea1nh v\u1edbi c\u1ed9t v\u00e0 di chuy\u1ec3n ch\u1eadm h\u01a1n. C\u00f2n c\u00e1c ph\u00e2n t\u1eed c\u00f3 \u00e1i l\u1ef1c m\u1ea1nh v\u1edbi pha \u0111\u1ed9ng s\u1ebd b\u1ecb k\u00e9o theo d\u00f2ng dung m\u00f4i v\u00e0 di chuy\u1ec3n nhanh h\u01a1n t\u1eeb \u0111\u00f3 ra kh\u1ecfi c\u1ed9t nhanh h\u01a1n nh\u01b0 h\u00ecnh d\u01b0\u1edbi \u0111\u00e2y.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"975\" height=\"524\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-1.png\" alt=\"\" class=\"wp-image-3580\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-1.png 975w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-1-300x161.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-1-768x413.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-1-18x10.png 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-1-600x322.png 600w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 2. <\/strong>Nguy\u00ean l\u00fd ho\u1ea1t \u0111\u1ed9ng, ph\u00e2n t\u00e1ch ch\u1ea5t c\u1ee7a HPLC<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">T\u00f9y thu\u1ed9c v\u00e0o b\u1ea3n ch\u1ea5t c\u1ee7a m\u1eabu c\u1ea7n ph\u00e2n t\u00edch, b\u1ea3n ch\u1ea5t c\u1ee7a pha t\u0129nh v\u00e0 c\u01a1 ch\u1ebf ph\u00e2n t\u00e1ch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng, c\u00f3 th\u1ec3 chia HPLC th\u00e0nh c\u00e1c lo\u1ea1i nh\u01b0 sau:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>HPLC pha thu\u1eadn<\/strong> <strong>(Normal-Phase HPLC)<\/strong>: S\u1eed d\u1ee5ng c\u00e1c pha t\u0129nh ph\u00e2n c\u1ef1c v\u00e0 pha \u0111\u1ed9ng kh\u00f4ng ph\u00e2n c\u1ef1c. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u00e1ch c\u00e1c h\u1ee3p ch\u1ea5t kh\u00f4ng ph\u00e2n c\u1ef1c.<\/li>\n\n\n\n<li><strong>HPLC pha \u0111\u1ea3o<\/strong> <strong>(Reverse-Phase HPLC):<\/strong> S\u1eed d\u1ee5ng pha t\u0129nh kh\u00f4ng ph\u00e2n c\u1ef1c v\u00e0 pha \u0111\u1ed9ng ph\u00e2n c\u1ef1c. \u0110\u00e2y l\u00e0 lo\u1ea1i s\u1eafc k\u00fd l\u1ecfng hi\u1ec7u su\u1ea5t cao \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i v\u00e0 ph\u1ed5 bi\u1ebfn nh\u1ea5t cho c\u00e1c h\u1ee3p ch\u1ea5t ph\u00e2n c\u1ef1c, ph\u00f9 h\u1ee3p v\u1edbi nhi\u1ec1u \u1ee9ng d\u1ee5ng ch\u1eb3ng h\u1ea1n nh\u01b0 trong ph\u00e2n t\u00edch d\u01b0\u1ee3c ph\u1ea9m v\u00e0 t\u00e1ch sinh h\u00f3a<\/li>\n\n\n\n<li><strong>S\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (Size Exclusion Chromatography)<\/strong>: c\u00e1c ph\u00e2n t\u1eed, c\u00e1c ch\u1ea5t \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc, v\u1edbi c\u00e1c ph\u00e2n t\u1eed c\u00f3 k\u00edch th\u01b0\u1edbc l\u1edbn h\u01a1n di chuy\u1ec3n nhanh h\u01a1n v\u00e0 r\u1eeda gi\u1ea3i ra tr\u01b0\u1edbc. N\u00f3 \u0111\u1eb7c bi\u1ec7t h\u1eefu \u00edch \u0111\u1ec3 ph\u00e2n t\u00edch polyme v\u00e0 protein.<\/li>\n\n\n\n<li><strong>S\u1eafc k\u00fd trao \u0111\u1ed5i ion (Ion-Exchange Chromatography)<\/strong>: qu\u00e1 tr\u00ecnh ph\u00e2n t\u00e1ch c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1a tr\u00ean s\u1ef1 trao \u0111\u1ed5i, t\u01b0\u01a1ng t\u00e1c \u0111i\u1ec7n t\u00edch gi\u1eefa ch\u1ea5t c\u1ea7n ph\u00e2n t\u00edch v\u00e0 pha t\u0129nh. Trong \u0111\u00f3 pha t\u0129nh th\u01b0\u1eddng mang nh\u00f3m mang \u0111i\u1ec7n t\u00edch tr\u00e1i d\u1ea5u v\u1edbi nh\u00f3m \u0111i\u1ec7n t\u00edch c\u1ee7a ch\u1ea5t c\u1ea7n ph\u00e2n t\u00e1ch.<\/li>\n\n\n\n<li><strong>S\u1eafc k\u00fd \u00e1i l\u1ef1c (Affinity Chromatograph)<\/strong>: k\u1ef9 thu\u1eadt c\u00f3 t\u00ednh \u0111\u1eb7c hi\u1ec7u cao n\u00e0y s\u1eed d\u1ee5ng c\u00e1c t\u00e1c nh\u00e2n c\u00f3 li\u00ean quan v\u1ec1 m\u1eb7t sinh h\u1ecdc (v\u00ed d\u1ee5: kh\u00e1ng th\u1ec3) l\u00e0m pha t\u0129nh \u0111\u1ec3 t\u00e1ch c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>5. \u1ee8ng d\u1ee5ng c\u1ee7a HPLC trong oligonucleotide<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u1ee8ng d\u1ee5ng c\u1ee7a HPLC trong c\u00e1c oligonucleotide n\u00f3i ri\u00eang v\u00e0 c\u00e1c s\u1ea3n ph\u1ea9m sinh h\u1ecdc n\u00f3i chung \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ee9ng minh l\u00e0 h\u1eefu \u00edch. Trong \u0111\u00f3, tinh s\u1ea1ch c\u00e1c s\u1ea3n ph\u1ea9m oligonucleotide v\u00e0 ph\u00e2n t\u00edch \u0111\u1ed9 tinh s\u1ea1ch oligonucleotide l\u00e0 2 \u1ee9ng d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t. Do b\u1ea3n ch\u1ea5t c\u1ee7a oligonucleotide l\u00e0 nh\u1eefng ph\u00e2n t\u1eed ph\u00e2n c\u1ef1c (\u01b0a n\u01b0\u1edbc) v\u00e0 mang \u0111i\u1ec7n t\u00edch \u00e2m \u1edf pH 7, n\u00ean ph\u01b0\u01a1ng ph\u00e1p th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 tinh s\u1ea1ch oligonucleotide hi\u1ec7n nay l\u00e0 Ion pair reverse-phase HPLC) v\u00e0 Ion-exchange HPLC. Qu\u00e1 tr\u00ecnh ch\u1ecdn<strong><a href=\"https:\/\/oligox.abtvn.com\/en\/phuong-phap-tinh-sach-oligonucleotide\/\" data-type=\"link\" data-id=\"https:\/\/oligox.abtvn.com\/phuong-phap-tinh-sach-oligonucleotide\/\"> ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch oligo <\/a><\/strong>b\u1eb1ng HPLC ph\u00f9 h\u1ee3p s\u1ebd t\u00f9y thu\u1ed9c v\u00e0o chi\u1ec1u d\u00e0i v\u00e0 \u0111\u1eb7c t\u00ednh c\u1ee7a oligo c\u1ea7n tinh s\u1ea1ch.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>5.1. HPLC pha \u0111\u1ea3o gh\u00e9p c\u1eb7p ion (Ion pair reverse-phase HPLC)<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao pha \u0111\u1ea3o gh\u00e9p c\u1eb7p ion (IP-RP-HPLC) c\u00f3 b\u1ea3n ch\u1ea5t l\u00e0 k\u1ef9 thu\u1eadt HPLC pha \u0111\u1ea3o nh\u01b0ng \u0111\u01b0\u1ee3c k\u1ebft h\u1ee3p b\u1ed5 sung th\u00eam c\u00e1c thu\u1ed1c th\u1eed gh\u00e9p c\u1eb7p ion trong pha \u0111\u1ed9ng \u0111\u1ec3 t\u0103ng c\u01b0\u1eddng kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch c\u00e1c h\u1ee3p ch\u1ea5t ion v\u00e0 ph\u00e2n c\u1ef1c t\u1eeb \u0111\u00f3 gi\u00fap t\u0103ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i, \u0111\u1ed9 tinh s\u1ea1ch c\u1ee7a s\u1ea3n ph\u1ea9m. C\u00e1c t\u00e1c nh\u00e2n gh\u00e9p c\u1eb7p ion th\u01b0\u1eddng c\u00f3 c\u1ea5u tr\u00fac chung g\u1ed3m: m\u1ed9t nh\u00f3m k\u1ef5 n\u01b0\u1edbc b\u00e1m v\u00e0o pha t\u0129nh v\u00e0 m\u1ed9t nh\u00f3m t\u00edch \u0111i\u1ec7n \u00e2m ho\u1eb7c d\u01b0\u01a1ng t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i t\u00e1c nh\u00e2n gh\u00e9p c\u1eb7p ion s\u1eed d\u1ee5ng. Do b\u1ea3n ch\u1ea5t c\u1ee7a c\u00e1c oligo l\u00e0 c\u00e1c ph\u00e2n t\u1eed mang \u0111i\u1ec7n t\u00edch <strong>\u00e2m<\/strong>, n\u00ean c\u00e1c ch\u1ea5t gh\u00e9p c\u1eb7p ion th\u01b0\u1eddng mang \u0111i\u1ec7n t\u00edch <strong>d\u01b0\u01a1ng<\/strong> c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng nh\u01b0 l\u00e0 TEA, DIEA, DMCHA nh\u01b0 h\u00ecnh b\u00ean d\u01b0\u1edbi \u0111\u00e2y.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img decoding=\"async\" width=\"975\" height=\"444\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-2.png\" alt=\"H\u00ecnh. C\u1ea5u tr\u00fac c\u1ee7a m\u1ed9t t\u00e1c nh\u00e2n gh\u00e9p c\u1eb7p ion d\u01b0\u01a1ng s\u1eed d\u1ee5ng trong IP-RP-HPLC cho tinh s\u1ea1ch oligo \" class=\"wp-image-3581\" style=\"width:696px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-2.png 975w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-2-300x137.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-2-768x350.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-2-18x8.png 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-2-600x273.png 600w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 3.<\/strong> C\u1ea5u tr\u00fac c\u1ee7a m\u1ed9t t\u00e1c nh\u00e2n gh\u00e9p c\u1eb7p ion d\u01b0\u01a1ng s\u1eed d\u1ee5ng trong IP-RP-HPLC cho tinh s\u1ea1ch oligo<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">C\u01a1 ch\u1ebf ph\u00e2n t\u00e1ch c\u1ee7a IP-RP-HPLC l\u00e0 d\u1ef1a tr\u00ean t\u00ednh k\u1ef5 n\u01b0\u1edbc v\u00e0 chi\u1ec1u d\u00e0i c\u1ee7a ph\u00e2n t\u1eed oligonucleotide c\u1ea7n tinh s\u1ea1ch. Nh\u1eefng ph\u00e2n t\u1eed oligonucleotide mang \u0111i\u1ec7n t\u00edch \u00e2m l\u00fac n\u00e0y s\u1ebd li\u00ean k\u1ebft v\u1edbi c\u00e1c ch\u1ea5t gh\u00e9p c\u1eb7p ion \u0111\u01b0\u1ee3c b\u1ed5 sung trong pha \u0111\u1ed9ng v\u00e0 nh\u1eefng ch\u1ea5t gh\u00e9p c\u1eb7p ion m\u1ed9t \u0111\u1ea7u c\u00f2n l\u1ea1i n\u00e0y s\u1ebd li\u00ean k\u1ebft v\u1edbi pha t\u0129nh (C\u1ed9t s\u1eafc k\u00fd C18 ho\u1eb7c C8). Nh\u1eefng ph\u00e2n t\u1eed oligo c\u00f3 t\u00ednh k\u1ef5 n\u01b0\u1edbc v\u00e0 c\u00f3 k\u00edch th\u01b0\u1edbc d\u00e0i c\u00f3 xu h\u01b0\u1edbng \u0111\u01b0\u1ee3c gi\u1eef l\u1ea1i l\u00e2u h\u01a1n, do oligonucleotide d\u00e0i h\u01a1n, mang nhi\u1ec1u nh\u00f3m phosphate (\u0111i\u1ec7n t\u00edch \u00e2m) h\u01a1n, v\u00e0 t\u00ednh k\u1ef5 n\u01b0\u1edbc c\u1ee7a oligo s\u1ebd li\u00ean k\u1ebft m\u1ea1nh h\u01a1n v\u1edbi pha t\u0129nh th\u00f4ng qua t\u01b0\u01a1ng t\u00e1c k\u1ef5 n\u01b0\u1edbc nh\u01b0 b\u1ea3n ch\u1ea5t c\u1ee7a k\u1ef9 thu\u1eadt n\u00e0y nh\u01b0 h\u00ecnh b\u00ean d\u01b0\u1edbi \u0111\u00e2y.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"975\" height=\"566\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-3.png\" alt=\"\" class=\"wp-image-3582\" style=\"width:622px;height:auto\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-3.png 975w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-3-300x174.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-3-768x446.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-3-18x10.png 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-3-600x348.png 600w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 4. <\/strong>Nguy\u00ean l\u00fd c\u1ee7a IP-RP-HPLC trong tinh s\u1ea1ch oligonucleotide<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">Hi\u1ec7n nay, \u0111\u00e2y l\u00e0 ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch hi\u1ec7u qu\u1ea3 \u0111\u1ed1i v\u1edbi c\u00e1c oligonucleotide bi\u1ebfn \u0111\u1ed5i c\u00f3 g\u1eafn hu\u1ef3nh quang (nh\u01b0 taqman probe) v\u00ec nh\u1eefng ph\u00e2n t\u1eed hu\u1ef3nh quang c\u00f3 t\u00ednh k\u1ef5 n\u01b0\u1edbc, t\u1eeb \u0111\u00f3 t\u1ea1o s\u1ef1 kh\u00e1c bi\u1ec7t gi\u1eefa tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau v\u00e0 tr\u00ecnh t\u1ef1 l\u1ed7i, l\u00e0m c\u01a1 s\u1edf gi\u00fap ph\u00e2n t\u00e1ch hi\u1ec7u qu\u1ea3 tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau kh\u1ecfi c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn v\u00e0 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i kh\u00f4ng g\u1eafn hu\u1ef3nh quang. H\u01a1n n\u1eefa, \u0111\u00e2y l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn \u0111\u1ec3 t\u1ed5ng h\u1ee3p quy m\u00f4 l\u1edbn do kh\u1ea3 n\u0103ng t\u1ef1 \u0111\u1ed9ng h\u00f3a c\u1ee7a h\u1ec7 th\u1ed1ng. Tuy nhi\u00ean, kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch d\u1ef1a tr\u00ean t\u00ednh k\u1ef5 n\u01b0\u1edbc c\u00f3 th\u1ec3 b\u1ecb \u1ea3nh h\u01b0\u1edfng theo chi\u1ec1u d\u00e0i c\u1ee7a oligonucleotide. Do \u0111\u00f3, k\u1ef9 thu\u1eadt n\u00e0y th\u01b0\u1eddng kh\u00f4ng \u0111\u01b0\u1ee3c khuy\u1ebfn ngh\u1ecb \u0111\u1ec3 tinh ch\u1ebf oligonucleotide >60 bases. M\u1eb7c d\u00f9 c\u00f3 th\u1ec3 tinh ch\u1ebf oligonucleotide d\u00e0i h\u01a1n (l\u00ean \u0111\u1ebfn 80 bases, trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p d\u00e0i h\u01a1n) b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, nh\u01b0ng \u0111\u1ed9 tinh khi\u1ebft v\u00e0 n\u0103ng su\u1ea5t c\u00f3 th\u1ec3 b\u1ecb \u1ea3nh h\u01b0\u1edfng. C\u00e1c s\u1ea3n ph\u1ea9m oligo sau khi tinh s\u1ea1ch b\u1eb1ng IP-RP-HPLC th\u01b0\u1eddng c\u00f3 \u0111\u1ed9 tinh khi\u1ebft >90% (th\u00f4ng qua ph\u00e2n t\u00edch HPLC).<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>5.2. S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng trao \u0111\u1ed5i ion (IE-HPLC)<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao trao \u0111\u1ed5i ion (IE-HPLC) (c\u1ee5 th\u1ec3 l\u00e0 trao \u0111\u1ed5i anion) d\u1ef1a tr\u00ean s\u1ed1 l\u01b0\u1ee3ng nh\u00f3m t\u00edch \u0111i\u1ec7n \u00e2m c\u1ee7a nh\u00f3m phosphate trong khung x\u01b0\u01a1ng ch\u00ednh c\u1ee7a oligonucleotide. Ph\u01b0\u01a1ng ph\u00e1p trao \u0111\u1ed5i anion li\u00ean quan \u0111\u1ebfn vi\u1ec7c s\u1eed d\u1ee5ng c\u00e1c pha \u0111\u1ed9ng c\u00f3 n\u1ed3ng \u0111\u1ed9 mu\u1ed1i cao nh\u01b0 Nacl tr\u00ean c\u00e1c c\u1ed9t c\u00f3 ch\u1ee9a c\u00e1c pha t\u0129nh amoni. C\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i c\u00f3 k\u00edch th\u01b0\u1edbc ng\u1eafn (shortmer) th\u01b0\u1eddng c\u00f3 s\u1ed1 l\u01b0\u1ee3ng \u0111i\u1ec7n t\u00edch \u00e2m \u00edt h\u01a1n so v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau do \u0111\u00f3 c\u00e1c tr\u00ecnh t\u1ef1 l\u1ed7i th\u01b0\u1eddng c\u00f3 li\u00ean k\u1ebft y\u1ebfu v\u1edbi c\u1ed9t n\u00ean \u0111\u01b0\u1ee3c r\u1eeda gi\u1ea3i s\u1edbm h\u01a1n so v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau . Tuy nhi\u00ean, IE-HPLC b\u1ecb gi\u1edbi h\u1ea1n b\u1edfi \u0111\u1ed9 d\u00e0i, th\u01b0\u1eddng kho\u1ea3ng 40 bases. C\u00e1c oligonucleotide d\u00e0i h\u01a1n d\u1eabn \u0111\u1ebfn kh\u1ea3 n\u0103ng ph\u00e2n t\u00e1ch th\u1ea5p h\u01a1n gi\u1eefa tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau v\u00e0 tr\u00ecnh t\u1ef1 l\u1ed7i; v\u00e0 do \u0111\u00f3, \u0111\u1ed9 tinh khi\u1ebft th\u01b0\u1eddng th\u1ea5p h\u01a1n v\u00e0 k\u00e9m nh\u1ea5t qu\u00e1n h\u01a1n.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"860\" height=\"601\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4.jpg\" alt=\"H\u00ecnh. Nguy\u00ean l\u00fd tinh s\u1ea1ch Oligo b\u1eb1ng IE-HPLC \" class=\"wp-image-3583\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4.jpg 860w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-300x210.jpg 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-768x537.jpg 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-18x12.jpg 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-600x419.jpg 600w\" sizes=\"(max-width: 860px) 100vw, 860px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 5. <\/strong>Nguy\u00ean l\u00fd tinh s\u1ea1ch Oligo b\u1eb1ng IE-HPLC<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>IEX-HPLC<\/strong> v\u00e0 <strong>IP-RP-HPLC<\/strong> \u0111\u1ec1u l\u00e0 hai ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch oligo hi\u1ec7u qu\u1ea3, nh\u01b0ng l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p n\u00e0o ph\u00f9 h\u1ee3p s\u1ebd t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i, \u0111\u1eb7c t\u00ednh v\u00e0 chi\u1ec1u d\u00e0i c\u1ee7a oligo.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em><strong>B\u1ea3ng 1. <\/strong>So s\u00e1nh gi\u1eefa ph\u01b0\u01a1ng ph\u00e1p IP-RP-HPLC v\u00e0 IE-HPLC<\/em><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Ti\u00eau ch\u00ed<\/strong><\/td><td><strong>IP-RP-HPLC <\/strong><strong><\/strong><\/td><td><strong>IE-HPLC<\/strong><strong><\/strong><\/td><\/tr><tr><td><strong>Nguy\u00ean l\u00fd t\u00e1ch<\/strong><\/td><td>D\u1ef1a tr\u00ean <strong>t\u01b0\u01a1ng t\u00e1c k\u1ef5 n\u01b0\u1edbc<\/strong> v\u00e0 pha t\u0129nh C18\/C8.<\/td><td>D\u1ef1a tr\u00ean <strong>t\u01b0\u01a1ng t\u00e1c \u0111i\u1ec7n t\u00edch<\/strong> gi\u1eefa nh\u00f3m phosphate (-PO\u2084\u00b3\u207b) c\u1ee7a oligo v\u00e0 pha t\u0129nh mang \u0111i\u1ec7n t\u00edch d\u01b0\u01a1ng.<\/td><\/tr><tr><td><strong>Pha t\u0129nh<\/strong><\/td><td>C\u1ed9t <strong>C18, C8<\/strong> k\u1ef5 n\u01b0\u1edbc.<\/td><td>C\u1ed9t s\u1eafc k\u00fd mang \u0111i\u1ec7n t\u00edch d\u01b0\u01a1ng<\/td><\/tr><tr><td><strong>Pha \u0111\u1ed9ng<\/strong><\/td><td>&#8211; Dung m\u00f4i h\u1eefu c\u01a1 (<strong>acetonitril<\/strong>) + ch\u1ea5t gh\u00e9p ion (TEAA, TBA).<\/td><td>&#8211; Dung d\u1ecbch \u0111\u1ec7m <strong>mu\u1ed1i t\u0103ng d\u1ea7n<\/strong> (NaCl, KCl) \u0111\u1ec3 c\u1ea1nh tranh v\u1edbi oligo.<\/td><\/tr><tr><td><strong>C\u01a1 ch\u1ebf gi\u1eef oligo<\/strong><\/td><td>&#8211; Oligo t\u1ef1 nhi\u00ean <strong>qu\u00e1 ph\u00e2n c\u1ef1c<\/strong>, kh\u00f4ng gi\u1eef \u0111\u01b0\u1ee3c tr\u00ean c\u1ed9t C18.<br>&#8211; Ch\u1ea5t gh\u00e9p c\u1eb7p ion gi\u00fap oligo t\u01b0\u01a1ng t\u00e1c v\u1edbi pha t\u0129nh.<\/td><td>&#8211; Oligo t\u00edch \u0111i\u1ec7n \u00e2m (-PO\u2084\u00b3\u207b) g\u1eafn v\u00e0o pha t\u0129nh t\u00edch \u0111i\u1ec7n d\u01b0\u01a1ng (c\u1ed9t trao \u0111\u1ed5i anion).<br>&#8211; Khi t\u0103ng n\u1ed3ng \u0111\u1ed9 mu\u1ed1i, c\u00e1c ion Cl\u207b c\u1ea1nh tranh v\u1edbi oligo v\u00e0 \u0111\u1ea9y n\u00f3 ra kh\u1ecfi c\u1ed9t.<\/td><\/tr><tr><td><strong>Chi\u1ec1u d\u00e0i khuy\u1ebfn ngh\u1ecb<\/strong><\/td><td>&lt; 60 bases<\/td><td>&lt; 50 bases<\/td><\/tr><tr><td><strong>Lo\u1ea1i oligo ph\u00f9 h\u1ee3p<\/strong><\/td><td>Ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c oligo bi\u1ebfn \u0111\u1ed5i c\u00f3 t\u00ednh k\u1ef5 n\u01b0\u1edbc nh\u01b0 taqman probe, primer c\u00f3 g\u1eafn hu\u1ef3nh quang<\/td><td>Ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c oligo d\u1ec5 t\u1ea1o c\u00e1c c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p nh\u01b0 RNA, c\u00e1c oligo c\u00f3 h\u00e0m l\u01b0\u1ee3ng %GC cao<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>6. Quy tr\u00ecnh tinh s\u1ea1ch oligonucleotide b\u1eb1ng HPLC<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">M\u1ed9t quy tr\u00ecnh tinh s\u1ea1ch oligo \u0111\u1eb7c tr\u01b0ng th\u01b0\u1eddng c\u00f3 nh\u1eefng b\u01b0\u1edbc ch\u00ednh nh\u01b0 sau bao g\u1ed3m:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 1: <\/strong>Chu\u1ea9n b\u1ecb m\u1eabu v\u00e0 buffer, dung m\u00f4i<br>&#8211; Ho\u00e0n nguy\u00ean c\u00e1c m\u1eabu oligo c\u1ea7n tinh s\u1ea1ch trong n\u01b0\u1edbc tinh khi\u1ebft HPLC ho\u1eb7c trong buffer HPLC \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng.<br>&#8211; L\u1ecdc m\u1eabu qua m\u00e0ng l\u1ecdc 0.22 \u00b5m \u0111\u1ec3 lo\u1ea1i b\u1ecf c\u00e1c c\u1eb7n t\u1ea1p ch\u1ea5t c\u00f3 th\u1ec3 l\u00e0m t\u1eafc c\u1ed9t HPLC.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 2: <\/strong>Chu\u1ea9n b\u1ecb dung m\u00f4i, buffer HPLC tinh s\u1ea1ch oligo<br>&#8211; L\u1ecdc c\u00e1c dung m\u00f4i v\u00e0 buffer s\u1eed d\u1ee5ng qua m\u00e0ng l\u1ecdc 0.22 \u00b5m \u0111\u1ec3 lo\u1ea1i b\u1ecf c\u00e1c t\u1ea1p ch\u1ea5t c\u00f3 th\u1ec3 l\u00e0m t\u1eafc ngh\u1ebdn c\u1ed9t HPLC<br>&#8211; \u0110\u00e1nh s\u00f3ng si\u00eau \u00e2m c\u00e1c dung m\u00f4i v\u00e0 buffer \u0111\u1ec3 lo\u1ea1i c\u00e1c b\u1ecdt kh\u00ed tr\u00e1nh \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 3:<\/strong> Chu\u1ea9n b\u1ecb c\u1ed9t v\u00e0 c\u00e0i \u0111\u1eb7t ch\u01b0\u01a1ng tr\u00ecnh h\u1ec7 th\u1ed1ng HPLC<br>&#8211; C\u1ed9t HPLC tr\u01b0\u1edbc khi s\u1eed d\u1ee5ng \u0111\u1ec3 tinh s\u1ea1ch s\u1ebd \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh c\u00e2n b\u1eb1ng c\u1ed9t trong 15 ph\u00fat<br>&#8211; Ch\u01b0\u01a1ng tr\u00ecnh h\u1ec7 th\u1ed1ng ch\u1ea1y HPLC th\u00f4ng th\u01b0\u1eddng s\u1ebd c\u00f3 nh\u1eefng th\u00f4ng s\u1ed1 c\u1ea7n ph\u1ea3i c\u00e0i \u0111\u1eb7t bao g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Nhi\u1ec7t \u0111\u1ed9: <\/strong>th\u00f4ng th\u01b0\u1eddng nhi\u1ec7t \u0111\u1ed9 h\u1ec7 th\u1ed1ng s\u1ebd \u0111\u01b0\u1ee3c c\u00e0i \u0111\u1eb7t \u1edf m\u1ee9c 60oC \u0111\u1ec3 ph\u00e1 v\u1ee1 c\u00e1c c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p c\u1ee7a oligonucleotide c\u00f3 th\u1ec3 h\u00ecnh th\u00e0nh t\u1eeb \u0111\u00f3 gi\u00fap qu\u00e1 tr\u00ecnh t\u00e1ch hi\u1ec7u qu\u1ea3 h\u01a1n<\/li>\n\n\n\n<li><strong>T\u1ed1c \u0111\u1ed9 d\u00f2ng: <\/strong>1 \u2013 10ml\/ph\u00fat t\u00f9y thu\u1ed9c v\u00e0o \u0111\u1eb7c \u0111i\u1ec3m c\u1ed9t HPLC v\u00e0 h\u1ec7 th\u1ed1ng HPLC \u0111ang s\u1eed d\u1ee5ng<\/li>\n\n\n\n<li><strong>B\u01b0\u1edbc s\u00f3ng ph\u00e1t hi\u1ec7n: <\/strong>260nm v\u00e0 280nm \u0111\u1ed1i v\u1edbi c\u00e1c oligo ti\u00eau chu\u1ea9n nh\u01b0 primer, ho\u1eb7c 260nm v\u00e0 b\u01b0\u1edbc s\u00f3ng hu\u1ef3nh quang \u0111\u1ed1i v\u1edbi c\u00e1c oligo bi\u1ebfn \u0111\u1ed5i c\u00f3 g\u1eafn hu\u1ef3nh quang nh\u01b0 taqman probe.<\/li>\n\n\n\n<li><strong>Ch\u01b0\u01a1ng<\/strong><strong> tr\u00ecnh <\/strong><strong>Gradient r\u1eeda gi\u1ea3i<\/strong>: Thay \u0111\u1ed5i t\u1ef7 l\u1ec7 gi\u1eefa c\u00e1c buffer, dung m\u00f4i \u0111\u1ec3 t\u1ea1o s\u1ef1 kh\u00e1c bi\u1ec7t gi\u00fap ph\u00e2n t\u00e1ch oligonucleotide m\u1ee5c ti\u00eau v\u1edbi c\u00e1c oligo b\u1ecb l\u1ed7i.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 4: \u00a0<\/strong>N\u1ea1p m\u1eabu v\u00e0 ph\u00e2n t\u00edch s\u01a1 b\u1ed9 \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh th\u1eddi gian l\u01b0u c\u1ee7a oligo c\u1ea7n tinh s\u1ea1ch<br>&#8211; C\u00e1c m\u1eabu oligo sau khi chu\u1ea9n b\u1ecb s\u1ebd \u0111\u01b0\u1ee3c ti\u00eam v\u00e0o h\u1ec7 th\u1ed1ng v\u00e0 ch\u1ea1y ch\u01b0\u01a1ng tr\u00ecnh \u0111\u00e3 c\u00e0i \u0111\u1eb7t tr\u01b0\u1edbc \u0111\u00f3.<br>&#8211; C\u00e1c oligo tr\u01b0\u1edbc khi tinh s\u1ea1ch th\u01b0\u1eddng s\u1ebd c\u00f3 m\u1ed9t s\u1ed1 s\u1ea3n ph\u1ea9m l\u1ed7i v\u1edbi c\u00e1c th\u1eddi gian l\u01b0u kh\u00e1c nhau nh\u01b0 h\u00ecnh b\u00ean d\u01b0\u1edbi \u0111\u00e2y\/ Trong \u0111\u00f3, c\u00e1c oligo m\u1ee5c ti\u00eau th\u01b0\u1eddng s\u1ebd c\u00f3 chi\u1ec1u cao peak l\u1edbn nh\u1ea5t v\u00e0 c\u00f3 xu h\u01b0\u1edbng ra tr\u1ec5 h\u01a1n so v\u1edbi c\u00e1c tr\u00ecnh t\u1ef1 oligo l\u1ed7i. Do \u0111\u00f3 l\u00fac n\u00e0y s\u1ebd ti\u1ebfn h\u00e0nh c\u00e0i \u0111\u1eb7t h\u1ec7 th\u1ed1ng \u0111\u1ec3 thu nh\u1eadn c\u00e1c ph\u00e2n \u0111o\u1ea1n, c\u00e1c peak \u1edf kho\u1ea3ng th\u1eddi gian t\u1eeb 25 &#8211; 29 ph\u00fat.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"921\" height=\"415\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4.png\" alt=\"H\u1ec9nh. K\u1ebft qu\u1ea3 s\u1ea3n ph\u1ea9m oligo tr\u01b0\u1edbc khi tinh s\u1ea1ch \" class=\"wp-image-3584\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4.png 921w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-300x135.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-768x346.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-18x8.png 18w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-4-600x270.png 600w\" sizes=\"(max-width: 921px) 100vw, 921px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 6. <\/strong>K\u1ebft qu\u1ea3 s\u1ea3n ph\u1ea9m oligo tr\u01b0\u1edbc khi tinh s\u1ea1ch<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 5: <\/strong>Lo\u1ea1i b\u1ecf dung m\u00f4i<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">&#8211; C\u00e1c oligo sau khi thu nh\u1eadn tr\u1ef1c ti\u1ebfp t\u1eeb m\u00e1y HPLC th\u01b0\u1eddng ch\u1ee9a c\u00e1c dung m\u00f4i h\u1eefu c\u01a1 do qu\u00e1 tr\u00ecnh tinh s\u1ea1ch HPLC, l\u00fac n\u00e0y c\u1ea7n ti\u1ebfn h\u00e0nh lo\u1ea1i b\u1ecf c\u00e1c dung m\u00f4i h\u1eefu c\u01a1 b\u1eb1ng c\u00e1c k\u1ef9 thu\u1eadt nh\u01b0 \u0111\u00f4ng kh\u00f4, ho\u1eb7c s\u1eed d\u1ee5ng speedvac.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 6: <\/strong>Ho\u00e0n nguy\u00ean oligo v\u00e0 ki\u1ec3m tra n\u1ed3ng \u0111\u1ed9 oligo<br>&#8211; C\u00e1c oligo sau khi \u0111\u01b0\u1ee3c \u0111\u00f4ng kh\u00f4 ho\u1eb7c l\u00e0m kh\u00f4 b\u1eb1ng speedvac s\u1ebd \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh ho\u00e0n nguy\u00ean trong dung d\u1ecbch TE \u0111\u1ec3 b\u1ea3o qu\u1ea3n v\u00e0 \u0111\u01b0\u1ee3c mang \u0111i \u0111o nanodrop \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh n\u1ed3ng \u0111\u1ed9 v\u00e0 h\u00f2a tan v\u1ec1 n\u1ed3ng \u0111\u1ed9 mong mu\u1ed1n.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u01b0\u1edbc 7: <\/strong>Ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng, \u0111\u1ed9 tinh khi\u1ebft c\u1ee7a oligo sau khi tinh s\u1ea1ch<br>&#8211; C\u00e1c s\u1ea3n ph\u1ea9m oligonucleotide sau khi tinh s\u1ea1ch s\u1ebd \u0111\u01b0\u1ee3c ki\u1ec3m tra, QC \u0111\u1ed9 tinh khi\u1ebft m\u1ed9t l\u1ea7n n\u1eefa b\u1eb1ng HPLC \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o ch\u1ea5t l\u01b0\u1ee3ng s\u1ea3n ph\u1ea9m t\u1ed1t nh\u1ea5t khi \u0111\u1ebfn tay kh\u00e1ch h\u00e0ng.<br>&#8211; C\u00e1c oligo sau khi tinh s\u1ea1ch s\u1ebd \u0111\u01b0\u1ee3c ki\u1ec3m tra \u0111\u1ed9 tinh khi\u1ebft \u1edf b\u01b0\u1edbc s\u00f3ng 260 nm v\u00e0 b\u01b0\u1edbc s\u00f3ng 280 ho\u1eb7c t\u00edn hi\u1ec7u hu\u1ef3nh quang t\u00f9y thu\u1ed9c v\u00e0o oligo s\u1ea3n xu\u1ea5t. \u0110\u1ed9 tinh khi\u1ebft c\u1ee7a s\u1ea3n ph\u1ea9m th\u00f4ng th\u01b0\u1eddng s\u1ebd \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh d\u1ef1a tr\u00ean k\u1ebft qu\u1ea3 s\u1eafc k\u00fd \u0111\u1ed3 b\u1eb1ng c\u00e1ch quan s\u00e1t s\u1ed1 l\u01b0\u1ee3ng peak c\u00f3 trong s\u1eafc k\u00fd \u0111\u1ed3 ho\u1eb7c c\u00f3 th\u1ec3 d\u1ef1a tr\u00ean th\u00f4ng s\u1ed1 % Area \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n tr\u00ean ph\u1ea7n m\u1ec1m h\u1ec7 th\u1ed1ng c\u1ee7a m\u00e1y HPLC.<br>&#8211; \u0110\u1ed9 tinh khi\u1ebft c\u1ee7a c\u00e1c s\u1ea3n ph\u1ea9m Oligo nh\u01b0 taqman probe t\u1eeb OligoX sau khi tinh s\u1ea1ch b\u1eb1ng HPLC lu\u00f4n cam k\u1ebft v\u00e0 \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 tinh khi\u1ebft > 90% cho s\u1ea3n ph\u1ea9m sau khi tinh s\u1ea1ch b\u1eb1ng HPLC nh\u01b0 h\u00ecnh b\u00ean d\u01b0\u1edbi \u0111\u00e2y.\u00a0<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"948\" height=\"713\" src=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-5.png\" alt=\"H\u00ecnh. K\u1ebft qu\u1ea3 s\u1ea3n ph\u1ea9m oligo sau khi tinh s\u1ea1ch b\u1eb1ng HPLC t\u1eeb OligoX\" class=\"wp-image-3585\" srcset=\"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-5.png 948w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-5-300x226.png 300w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-5-768x578.png 768w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-5-16x12.png 16w, https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/image-5-600x451.png 600w\" sizes=\"(max-width: 948px) 100vw, 948px\" \/><figcaption class=\"wp-element-caption\"><em><strong>H\u00ecnh 7. <\/strong>K\u1ebft qu\u1ea3 s\u1ea3n ph\u1ea9m oligo sau khi tinh s\u1ea1ch b\u1eb1ng HPLC t\u1eeb OligoX<\/em><\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">7. <strong>T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>HPLC complete guide: All about History, Definition, Principle, Types, Instrumentation, and applications. (2022, March 28).\u00a0<em>Tech-Publish<\/em>. <a href=\"https:\/\/tech-publish.com\/hplc-guide-history-definition-principle-types-instrumentation\/\" target=\"_blank\" rel=\"noopener\">https:\/\/tech-publish.com\/hplc-guide-history-definition-principle-types-instrumentation\/<\/a><\/li>\n\n\n\n<li>Science Unfiltered. (2020, June 23). Synthetic oligonucleotides &#8211; analysis of their complex structure.\u00a0SCIENCE UNFILTERED. <a href=\"https:\/\/phenomenex.blog\/2020\/06\/23\/synthetic-oligonucleotides\/\" target=\"_blank\" rel=\"noopener\">https:\/\/phenomenex.blog\/2020\/06\/23\/synthetic-oligonucleotides\/<\/a><\/li>\n\n\n\n<li>Vcr V.-T. b\u1ecb P. (2024, January 11).\u00a0HPLC l\u00e0 g\u00ec? \u1ee8ng d\u1ee5ng c\u1ee7a HPLC trong ki\u1ec3m nghi\u1ec7m thu\u1ed1c. Vietnamcleanroom &#8211; Thi\u1ebft b\u1ecb ph\u00f2ng s\u1ea1ch VCR. <a href=\"https:\/\/vietnamcleanroom.com\/vi\/post\/hplc-la-gi--ung-dung-cua-hplc-trong-kiem-nghiem-thuoc-1455.htm\" target=\"_blank\" rel=\"noopener\">https:\/\/vietnamcleanroom.com\/vi\/post\/hplc-la-gi&#8211;ung-dung-cua-hplc-trong-kiem-nghiem-thuoc-1455.htm<\/a><\/li>\n\n\n\n<li>(N.d.-a). Retrieved February 13, 2025, from<a href=\" http:\/\/bahttps:\/\/www.shimadzu.com\/an\/service-support\/technical-support\/analysis-basics\/basic\/what_is_hplc.html\"> http:\/\/bahttps:\/\/www.shimadzu.com\/an\/service-support\/technical-support\/analysis-basics\/basic\/what_is_hplc.html<\/a><\/li>\n\n\n\n<li>(N.d.-b). Sigmaaldrich.com. Retrieved February 13, 2025, from <a href=\"https:\/\/www.sigmaaldrich.com\/VN\/en\/technical-documents\/technical-article\/genomics\/dna-and-rna-purification\/custom-dna-oligos-purity-verification\" target=\"_blank\" rel=\"noopener\">https:\/\/www.sigmaaldrich.com\/VN\/en\/technical-documents\/technical-article\/genomics\/dna-and-rna-purification\/custom-dna-oligos-purity-verification<\/a><\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>HPLC (High Performance Liquid Chromatography) \u2013 S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch, \u0111\u1ecbnh t\u00ednh ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng c\u00e1c ch\u1ea5t, th\u00e0nh ph\u1ea7n trong m\u1ed9t h\u1ed7n h\u1ee3p ch\u1ea5t l\u1ecfng.<\/p>","protected":false},"author":1,"featured_media":3591,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[23,1,28],"tags":[29,44],"class_list":["post-3578","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bai-viet","category-uncategorized","category-application","tag-oligonucleotide","tag-tinh-sach-oligo"],"jetpack_featured_media_url":"https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC.webp","uagb_featured_image_src":{"full":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC.webp",1920,1080,false],"thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-150x150.webp",150,150,true],"medium":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-300x169.webp",300,169,true],"medium_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-768x432.webp",768,432,true],"large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-1024x576.webp",1024,576,true],"1536x1536":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-1536x864.webp",1536,864,true],"2048x2048":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC.webp",1920,1080,false],"trp-custom-language-flag":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-18x10.webp",18,10,true],"ultp_layout_landscape_large":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-1200x800.webp",1200,800,true],"ultp_layout_landscape":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-870x570.webp",870,570,true],"ultp_layout_portrait":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-600x900.webp",600,900,true],"ultp_layout_square":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-600x600.webp",600,600,true],"woocommerce_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-300x300.webp",300,300,true],"woocommerce_single":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-600x338.webp",600,338,true],"woocommerce_gallery_thumbnail":["https:\/\/oligox.abtvn.com\/wp-content\/uploads\/2025\/02\/ABT-HPLC-100x100.webp",100,100,true]},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/oligox.abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":1,"uagb_excerpt":"HPLC (High Performance Liquid Chromatography) \u2013 S\u1eafc k\u00fd l\u1ecfng hi\u1ec7u n\u0103ng cao l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch, \u0111\u1ecbnh t\u00ednh ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng c\u00e1c ch\u1ea5t, th\u00e0nh ph\u1ea7n trong m\u1ed9t h\u1ed7n h\u1ee3p ch\u1ea5t l\u1ecfng.","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3578","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=3578"}],"version-history":[{"count":3,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3578\/revisions"}],"predecessor-version":[{"id":3592,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/posts\/3578\/revisions\/3592"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/media\/3591"}],"wp:attachment":[{"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=3578"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=3578"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/oligox.abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=3578"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}